Variations in the Intragene Methylation Profiles Hallmark Induced Pluripotency.

We demonstrate the potential of differentiating embryonic and induced pluripotent stem cells by the regularized linear and decision tree machine learning classification algorithms, based on a number of intragene methylation measures. The resulting average accuracy of classification has been proven t...

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Publicado en:BioMed Research International Vol. 2015; pp. 1 - 10
Autores principales: Druzhkov, Pavel, Zolotykh, Nikolay, Meyerov, Iosif, Alsaedi, Ahmed, Shutova, Maria, Ivanchenko, Mikhail, Zaikin, Alexey
Formato: equations & formulas research tables/charts Journal Article
Publicado: Wiley-Blackwell 11/5/2015
Acceso en línea:Ver este registro en EBSCOhost
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      dt: 11/5/2015
      vid: 2015
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      pub: Wiley-Blackwell
      place: Malden, Massachusetts
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        10.1155/2015/976362
        113630016
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        atl: Variations in the Intragene Methylation Profiles Hallmark Induced Pluripotency.
      aug:
        au:
          Druzhkov, Pavel
          Zolotykh, Nikolay
          Meyerov, Iosif
          Alsaedi, Ahmed
          Shutova, Maria
          Ivanchenko, Mikhail
          Zaikin, Alexey
        affil: Department of Algebra, Geometry and Discrete Mathematics, Lobachevsky State University of Nizhny Novgorod, Nizhny Novgorod, Russia
      sug:
        subj:
          Stem Cells Classification
          Algorithms
          DNA Methylation
          Human
          Decision Trees
          Phenotype
      ab: We demonstrate the potential of differentiating embryonic and induced pluripotent stem cells by the regularized linear and decision tree machine learning classification algorithms, based on a number of intragene methylation measures. The resulting average accuracy of classification has been proven to be above 95%, which overcomes the earlier achievements. We propose a constructive and transparent method of feature selection based on classifier accuracy. Enrichment analysis reveals statistically meaningful presence of stemness group and cancer discriminating genes among the selected best classifying features. These findings stimulate the further research on the functional consequences of these differences in methylation patterns. The presented approach can be broadly used to discriminate the cells of different phenotype or in different state by their methylation profiles, identify groups of genes constituting multifeature classifiers, and assess enrichment of these groups by the sets of genes with a functionality of interest.
      pubtype: Academic Journal
      doctype:
        equations & formulas
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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