Proapoptotic effect of control-released basic fibroblast growth factor on skin wound healing in a diabetic mouse model.
The ability of basic fibroblast growth factor (bFGF) to improve wound healing is attenuated by its short half-life in free form. This study aimed to enhance skin wound healing in a diabetes mouse model while concomitantly decreasing scar formation using control-released bFGF together with acidic gel...
| Publicado en: | Wound Repair & Regeneration Vol. 24; no. 1; pp. 65 - 75 |
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| Autores principales: | , , , , , , |
| Formato: | equations & formulas pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
Jan/Feb2016
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=113894761&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 113894761 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 10671927 DPV jtl: Wound Repair & Regeneration issn: 10671927 maglogo: Y pubinfo: dt: Jan/Feb2016 vid: 24 iid: 1 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 113894761 113894761 113894761 10.1111/wrr.12375 113894761 ppf: 65 ppct: 10 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Proapoptotic effect of control-released basic fibroblast growth factor on skin wound healing in a diabetic mouse model. aug: au: Huang, Chenyu Orbay, Hakan Tobita, Morikuni Miyamoto, Masaaki Tabata, Yasuhiko Hyakusoku, Hiko Mizuno, Hiroshi affil: Department of Plastic, Reconstructive and Aesthetic Surgery, Nippon Medical School, Tokyo, Japan sug: subj: Diabetes Mellitus Wound Care Methods Growth Substances Administration and Dosage Fibroblasts Gels Administration and Dosage Cicatrix Prevention and Control Wound Healing Animal Studies In Vivo Studies Mice Funding Source Histological Techniques Endothelium Analysis Neovascularization, Physiologic Evaluation Immunohistochemistry Apoptosis Evaluation Staining and Labeling Time Factors Delayed-Action Preparations Therapeutic Use Descriptive Statistics P-Value Japan Image Processing, Computer Assisted Data Analysis Software Mathematics Microscopy Two-Tailed Test Analysis of Variance ab: The ability of basic fibroblast growth factor (bFGF) to improve wound healing is attenuated by its short half-life in free form. This study aimed to enhance skin wound healing in a diabetes mouse model while concomitantly decreasing scar formation using control-released bFGF together with acidic gelatin hydrogel microspheres (AGHMs). Bilateral full-thickness wounds (10 mm in diameter) were made on the backs of db/db mice. Forty-five mice were divided into three groups, and the base of the wound under the panniculus carnosus and the wound periphery were injected with phosphate-buffered saline (300 μL) containing (1) controlreleased bFGF (50 μg), (2) control-released bFGF (20 μg), or (3) AGHMs alone. The size of the wound area was recorded on each postoperative day (POD). Mice were sacrificed on postoperative day 4, 7, 10, 14, and 28, and skin wound specimens were obtained to assess the endothelium/angiogenesis index via cluster of differentiation 31 immunohistochemistry, the proliferation index via Ki-67 immunohistochemistry, and the myofibroblast and fibroblast apoptosis indices by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling and alphasmooth muscle actin or vimentin staining, respectively. Epithelialization rates and indices of proliferation and myofibroblast/fibroblast apoptosis were higher in the bFGF groups than in the AGHM group, mainly within 2 weeks of injury. No dose-effect relationship was found for control-released bFGF, although the actions of 50 μg bFGF seemed to last longer than those of 20 μg bFGF. Therefore, control-released bFGF may accelerate diabetic skin wound healing and induce myofibroblast/fibroblast apoptosis, thereby reducing scar formation. pubtype: Academic Journal doctype: equations & formulas pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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