The natural behavior of mononuclear phagocytes in HTS formation.
Hypertrophic scars (HTS) are caused by trauma or burn injuries to the deep dermis and are considered fibrosis in the skin. Monocytes, M1 and M2 macrophages are mononuclear phagocytes. Studies suggest that M2 macrophages are profibrotic and might contribute to HTS formation. Our lab has established a...
| Publicado en: | Wound Repair & Regeneration Vol. 24; no. 1; pp. 14 - 26 |
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| Autores principales: | , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
Jan/Feb2016
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=113894763&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 113894763 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 10671927 DPV jtl: Wound Repair & Regeneration issn: 10671927 maglogo: Y pubinfo: dt: Jan/Feb2016 vid: 24 iid: 1 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 113894763 113894763 113894763 10.1111/wrr.12378 113894763 ppf: 14 ppct: 12 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: The natural behavior of mononuclear phagocytes in HTS formation. aug: au: Zhu, Zhensen Ding, Jie Ma, Zengshuan Iwashina, Takashi Tredget, Edward E affil: Division of Plastic and Reconstructive Surgery, Wound Healing Research Group, University of Alberta, Edmonton, Alberta, Canada sug: subj: Phagocytes Physiology Skin Pathology Fibrosis Funding Source Models, Biological Animal Studies In Vivo Studies Mice Human Skin Transplantation Histological Techniques Collagen Analysis Fibroblasts Analysis Macrophages Analysis Monocytes Analysis Xenografts Phenotype Evaluation Cicatrix, Hypertrophic Female Digital Imaging Staining and Labeling Immunochemistry Flow Cytometry Reverse Transcriptase Polymerase Chain Reaction Descriptive Statistics Data Analysis Software Two-Tailed Test Unpaired T-Tests One-Way Analysis of Variance Post Hoc Analysis P-Value Female ab: Hypertrophic scars (HTS) are caused by trauma or burn injuries to the deep dermis and are considered fibrosis in the skin. Monocytes, M1 and M2 macrophages are mononuclear phagocytes. Studies suggest that M2 macrophages are profibrotic and might contribute to HTS formation. Our lab has established a human HTS-like nude mouse model, in which the grafted human skin develops red, raised, and firm scarring, resembling HTS seen in humans. In this study, we observed the natural behavior of mononuclear phagocyte system in this nude mouse model of dermal fibrosis at multiple time points. Thirty athymic nude mice received human skin grafts and an equal number of mice received mouse skin grafts as controls. The grafted skin and blood were harvested at 1, 2, 3, 4, and 8 weeks. Wound area, thickness, collagen morphology and level, the cell number of myofibroblasts, M1- and M2-like macrophages in the grafted skin, as well as monocyte fraction in the blood were investigated at each time points. Xenografted mice developed contracted and thickened scars grossly. The xenografted skin resembled human HTS tissue based on enhanced thickness, fibrotic orientation of collagen bundles, increased collagen level, and infiltration of myofibroblasts. In the blood, monocytes dramatically decreased at 1 week postgrafting and gradually returned to normal in the following 8 weeks. In the xenografted skin, M1-like macrophages were found predominantly at 1-2 weeks postgrafting; whereas, M2-like macrophages were abundant at later time points, 3-4 weeks postgrafting coincident with the development of fibrosis in the human skin tissues. This understanding of the natural behavior of mononuclear phagocytes in vivo in our mouse model provides evidence for the role of M2- like macrophages in fibrosis of human skin and suggests that macrophage depletion in the subacute phases of wound healing might reduce or prevent HTS formation. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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