Study of Biofilm Formation in C57BL/6J Mice by Clinical Isolates of Helicobacter pylori.
Background/Aim: Despite the significant number of studies on H. pylori pathogenesis, not much data has been published concerning its ability to form biofilm in the host stomach. This study aims to evaluate the potential of clinical isolates of H. pylori to form biofilm in C57BL/ 6J mice model. Mater...
| Publicado en: | Saudi Journal of Gastroenterology Vol. 31; no. 2; pp. 161 - 169 |
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| Autores principales: | , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Wolters Kluwer India Pvt Ltd
Apr-Jun2016
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=113935673&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 113935673 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 13193767 322P jtl: Saudi Journal of Gastroenterology issn: 13193767 maglogo: N pubinfo: dt: Apr-Jun2016 vid: 31 iid: 2 pid: 16919 pub: Wolters Kluwer India Pvt Ltd artinfo: ui: 113935673 113935673 113935673 10.4103/1319-3767.178529 113935673 ppf: 161 ppct: 8 formats: tig: atl: Study of Biofilm Formation in C57BL/6J Mice by Clinical Isolates of Helicobacter pylori. aug: au: Attaran, Bahareh Falsafi, Tahereh Moghaddam, Ali N. affil: Department of Microbiology, School of Biology, Alzahra University sug: subj: Biofilms Helicobacter Infections Pathology Stomach Microbiology Mice Animal Studies Staining and Labeling Gastrostomy Tubes Bacterial Colonization Urease Enzyme-Linked Immunosorbent Assay Bacterial Infections Pathology Fluorescent Antibody Technique Microscopy, Electron, Scanning In Vitro Studies Immunoglobulins Epithelium Pathology Gastrointestinal System Pathology Gastric Mucosa Microbiology ab: Background/Aim: Despite the significant number of studies on H. pylori pathogenesis, not much data has been published concerning its ability to form biofilm in the host stomach. This study aims to evaluate the potential of clinical isolates of H. pylori to form biofilm in C57BL/ 6J mice model. Materials and Methods: Two strains of H. pylori were selected from a collection of clinical isolates; one (19B), an efficient biofilm producer and the other (4B), with weak biofilm-forming ability. Mice infected through gastric gavages were examined after one and two weeks. Colonization was determined by CFU and urease activity; the anti-H. pylori IgA was measured by ELISA, and chronic infections were evaluated by histopathology. Bacterial communities within mucosal sections were studied by immunofluorescence and scanning electron microscopy (SEM). Results: Successful infection was obtained by both test strains. Strain 19B with higher ability to form biofilm in vitro also showed a higher colonization rate in the mice stomach one week after infection. Difference (P < 0.05) in IgA titers was observed between the infected mice and the controls as well as between 19B and 4B infected mice, two weeks after the last challenge. Immunofluorescence and SEM results showed tightly colonizing H. pylori in stomach mucosal sections and in squamous and glandular epithelium. Conclusion: H. pylori is able to form biofilm in the mouse stomach and induce IgA production, reflecting the same potential as in humans. Firm attachment of coccoid form bacteria to host cells suggests the importance of this state in biofilm formation by H. pylori. Occurrence of biofilm in squamous and glandular epithelium of the mouse stomach proposes that H. pylori can colonize all parts of the upper gastrointestinal tract. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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