Detection of Helicobacter pylori DNA in Some Egyptian Water Systems and Its Incidence of Transmission to Individuals.
Background: The current study aimed to detect the presence of Helicobacter pylori in some water systems in Egypt as well as in blood samples of patients suffering from stomach ulcers. Methods: Fifty-one water samples collected from some Egyptian governorates; Giza, Alexandria, Monofia, Beheira, Miny...
| Publicado en: | Iranian Journal of Public Health Vol. 44; no. 2; pp. 203 - 211 |
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| Autores principales: | , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Tehran University of Medical Sciences
Feb2015
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=114008752&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 114008752 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 22516085 6N6F jtl: Iranian Journal of Public Health issn: 22516085 maglogo: N pubinfo: dt: Feb2015 vid: 44 iid: 2 pid: 21783 pub: Tehran University of Medical Sciences artinfo: ui: 114008752 114008752 114008752 114008752 ppf: 203 ppct: 8 formats: fmt: @attributes: type: P tig: atl: Detection of Helicobacter pylori DNA in Some Egyptian Water Systems and Its Incidence of Transmission to Individuals. aug: au: EL-SHAROUNY, Ebaa EL-SHAZLI, Hossam OLAMA, Zakia affil: Botany and Microbiology Department, Faculty of Science, Alexandria University, Alexandria, Egypt sug: subj: Water Analysis Helicobacter Pylori DNA Water Pollution Helicobacter Infections Blood Egypt Water Microbiology Human Peptic Ulcer Etiology Urease Enzyme-Linked Immunosorbent Assay Descriptive Statistics Developing Countries Chi Square Test Data Analysis Software Helicobacter Infections Transmission Polymerase Chain Reaction ab: Background: The current study aimed to detect the presence of Helicobacter pylori in some water systems in Egypt as well as in blood samples of patients suffering from stomach ulcers. Methods: Fifty-one water samples collected from some Egyptian governorates; Giza, Alexandria, Monofia, Beheira, Minya, Sohag, was subjected to physical and chemical analysis. Urease gene ureC, a highly specific sequence in H. pylori DNA was tracked in tested water samples and then in blood samples of patients' resident in areas with infected water supply using PCR technique. Data were all fed to the computer using IBM SPSS software package version 20.0. Qualitative data were described using number and percent. Comparison between different groups regarding categorical variables was tested using Chi-square test. Results: Two samples, belonging to Abu El Matamir-Beheira, and Sidi Bishr-Alexandria, showed positive results for presence of H. pylori. These two samples gave a positive result for the second time, on applying PCR technique for the cultures isolated under microaeroplilic conditions. Blood samples (173) were withdrawn from individuals living in the two areas with infected water supply. As diagnosed by ELISA, positive tests for H. pylori were recorded in 33.3% and 33.8% of patients suffering from ulcers in Abu El Matamir-Beheira, and Sidi Bishr-Alexandria, respectively. Conclusion: In developing countries such as Egypt, there is a big possibility for H. pylori to be transmitted via drinking water, hence, causing an epidemic infection, particularly in dense populated areas. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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