Modulation of cholesterol-related gene expression by ergosterol and ergosterol-enriched extracts obtained from Agaricus bisporus.
Purpose: To investigate the effect of two extracts obtained from Agaricus bisporus on the mRNA expression of cholesterol-related genes. One of the extracts contained ergosterol and other fungal sterols (SFE) and the other contained β-glucans and fungal sterols (EβG). Methods: Firstly, the dietary mi...
| Publicado en: | European Journal of Nutrition Vol. 55; no. 3; pp. 1041 - 1058 |
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| Autores principales: | , , , , , , , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Springer Nature
Apr2016
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=114190404&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 114190404 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 14366207 CR0 jtl: European Journal of Nutrition issn: 14366207 maglogo: N pubinfo: dt: Apr2016 vid: 55 iid: 3 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 114190404 114190404 114190404 10.1007/s00394-015-0918-x 114190404 ppf: 1041 ppct: 17 formats: fmt: @attributes: type: P tig: atl: Modulation of cholesterol-related gene expression by ergosterol and ergosterol-enriched extracts obtained from Agaricus bisporus. aug: au: Gil-Ramírez, Alicia Caz, Víctor Martin-Hernandez, Roberto Marín, Francisco Largo, Carlota Rodríguez-Casado, Arantxa Tabernero, María Ruiz-Rodríguez, Alejandro Reglero, Guillermo Soler-Rivas, Cristina affil: Department of Production and Characterization of Novel Foods, CIAL - Research Institute in Food Science (UAM+CSIC), Universidad Autónoma de Madrid, C/Nicolas Cabrera 9, Campus de Cantoblanco 28049 Madrid Spain sug: subj: Mushroom, Edible Genes Physiology Cholesterol Metabolism Phytosterols Pharmacodynamics Human Spain Funding Source In Vitro Studies Models, Biological Biological Assay In Vivo Studies Animal Studies Mice Cholesterol Blood Feces Analysis Liver Analysis Histological Techniques Dietary Supplementation Glucans Pharmacodynamics Descriptive Statistics Data Analysis Software One-Way Analysis of Variance Post Hoc Analysis P-Value ab: Purpose: To investigate the effect of two extracts obtained from Agaricus bisporus on the mRNA expression of cholesterol-related genes. One of the extracts contained ergosterol and other fungal sterols (SFE) and the other contained β-glucans and fungal sterols (EβG). Methods: Firstly, the dietary mixed micelles (DMMs) generated after in vitro digestion of standards and SFE were applied to Caco2 cells. Then, the lower compartment after a Caco2-transport experiment was applied to HepG2 cells. The mRNA expression was assessed in both cell lines by low-density arrays (LDA). Mice received the extracts, ergosterol or control drugs after 4 weeks of a high-cholesterol diet. The lipid profile of plasma, liver and feces was determined. LDA assays were performed in liver and intestines. Results: The DMM fraction of SFE up-regulated the LDLR mRNA expression in Caco2 cells. The lower compartment after Caco2-transport experiments up-regulated LDLR and modulated several other lipid-related genes in HepG2 cells. In mice, SFE decreased TC/HDL ratio and reduced hepatic triglycerides paralleled with down-regulation of Dgat1 expression, while EβG did it without transcriptional changes. Addition of SFE or ergosterol induced in jejunum a similar transcriptional response to simvastatin and ezetimibe; they all down-regulated Srebf2 and Nr1h4 (FXR) genes. Conclusion: Ergosterol-containing extracts from A. bisporus lowered hepatic triglyceride and modify the mRNA expression of cholesterol-related genes although the transcriptional regulation was unrelated to changes in plasma lipid profile. These extracts may be useful limiting hepatic steatosis and as bioactive ingredients to design novel functional foods preventing lifestyle-related diseases such as non-alcoholic fatty liver disease. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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