Multiplatform metabolomic fingerprinting as a tool for understanding hypercholesterolemia in Wistar rats.
Purpose: The aim was to investigate the impact of hypercholesterolemic diet on the metabolome of male Wistar rats by a multiplatform metabolomic fingerprinting. Methods: Male Wistar rats were fed with two different diets [control (C) and high-cholesterol diet (HC)-containing 2 % cholesterol and 0.5...
| Published in: | European Journal of Nutrition Vol. 55; no. 3; pp. 997 - 1011 |
|---|---|
| Main Authors: | , , , , , , |
| Format: | research tables/charts Journal Article |
| Published: |
Springer Nature
Apr2016
|
| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=114190408&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 114190408 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 14366207 CR0 jtl: European Journal of Nutrition issn: 14366207 maglogo: N pubinfo: dt: Apr2016 vid: 55 iid: 3 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 114190408 114190408 114190408 10.1007/s00394-015-0914-1 114190408 ppf: 997 ppct: 14 formats: fmt: @attributes: type: P tig: atl: Multiplatform metabolomic fingerprinting as a tool for understanding hypercholesterolemia in Wistar rats. aug: au: González-Peña, Diana Dudzik, Danuta Colina-Coca, Clara Ancos, Begoña García, Antonia Barbas, Coral Sánchez-Moreno, Concepción affil: Institute of Food Science, Technology and Nutrition (ICTAN), Spanish National Research Council (CSIC), José Antonio Novais 10 28040 Madrid Spain sug: subj: Cholesterol, Dietary Administration and Dosage Hypercholesterolemia Metabolism Spain Animal Studies In Vivo Studies Rats Funding Source Cholesterol, Dietary Pharmacodynamics Histological Techniques Gas Chromatography-Mass Spectrometry Chromatography, Liquid Mass Spectrometry Multivariate Analysis Data Analysis Software P-Value Descriptive Statistics Univariate Statistics T-Tests Confidence Intervals Electrophoresis Food Intake Evaluation Weight Gain Evaluation Lipids Metabolism Energy Metabolism Signal Transduction Amino Acids Metabolism Biochemical Phenomena ab: Purpose: The aim was to investigate the impact of hypercholesterolemic diet on the metabolome of male Wistar rats by a multiplatform metabolomic fingerprinting. Methods: Male Wistar rats were fed with two different diets [control (C) and high-cholesterol diet (HC)-containing 2 % cholesterol and 0.5 % cholic acid]. After 7 weeks of experimental feeding, the rats were euthanized for blood collection and plasma recovery. The metabolite fingerprint was then achieved by applying a multiplatform comprising LC-MS, GC-MS and CE-MS. Results: Multivariate statistical analysis showed a clear separation between the C and HC groups. Individual differences in metabolites were evaluated using univariate statistical analysis, and multiple metabolites were identified and confirmed in the plasma. A global profiling integrates for the first time pathways affected by high-cholesterol diet intake and allowed us to elucidate some of the associated alterations underlying the hypercholesterolemia event in Wistar rats. Conclusions: HC feeding stimulated the alteration of multiple pathways in Wistar rats, warning of the risk of developing important diseases, which can be modulated by the diet. Further studies are required to investigate the possibilities to revert or ameliorate the negative effects triggered by HC intake. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|