A novel combination of drug therapy to protect residual hearing post cochlear implant surgery.
ConclusionsA cocktail combining NAC, Mannitol, and Dexamethasone may be used to prevent loss of residual hearing post-implantation. There is a window of opportunity to treat the cochlea before the onset of cell death in HCs.ObjectiveInner ear trauma caused by cochlear implant electrode insertion tra...
| Publicado en: | Acta Oto-Laryngologica Vol. 136; no. 4; pp. 420 - 425 |
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| Autores principales: | , , , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Taylor & Francis Ltd
Apr2016
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=114327417&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 114327417 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00016489 B6D jtl: Acta Oto-Laryngologica issn: 00016489 maglogo: Y pubinfo: dt: Apr2016 vid: 136 iid: 4 pid: 377 pub: Taylor & Francis Ltd place: Philadelphia, Pennsylvania artinfo: ui: 114327417 114327417 114327417 10.3109/00016489.2015.1134809 114327417 ppf: 420 ppct: 5 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: A novel combination of drug therapy to protect residual hearing post cochlear implant surgery. aug: au: Eshraghi, Adrien A. Roell, Jonathan Shaikh, Noah Telischi, Fred F. Bauer, Blake Guardiola, Mateo Bas, Esperanza Van De Water, Thomas Rivera, Ileana Mittal, Jeenu affil: Department of Otolaryngology, University of Miami Miller School of Medicine, Miami, FL, USA sug: subj: Drug Therapy, Combination Cochlear Implant Hearing Disorders Prevention and Control Hearing Disorders Drug Therapy Postoperative Care Mannitol Administration and Dosage Dexamethasone Administration and Dosage Acetylcysteine Administration and Dosage Animal Studies Rats Tissue Culture Techniques In Vitro Studies Models, Biological Hair Cells Drug Effects Staining and Labeling Reactive Oxygen Species Microscopy Methods Descriptive Statistics Data Analysis Software ab: ConclusionsA cocktail combining NAC, Mannitol, and Dexamethasone may be used to prevent loss of residual hearing post-implantation. There is a window of opportunity to treat the cochlea before the onset of cell death in HCs.ObjectiveInner ear trauma caused by cochlear implant electrode insertion trauma (EIT) initiates multiple molecular mechanisms in hair cells (HCs) or support cells (SCs), resulting in initiation of programmed cell death within the damaged tissues of the cochlea, which leads to loss of residual hearing. In earlier studies L-N-acetylcysteine (L-NAC), Mannitol, and dexamethasone have been shown independently to protect the HCs loss against different types of inner ear trauma. These three molecules have different otoprotective effects. The goal of this preliminary study is to test the efficacy of a combination of these molecules to enhance the otoprotection of HCs against EIT.MethodsOC explants were dissected from P-3 rats and placed in serum-free media. Explants were divided into control and experimental groups. Control group: (1) untreated controls; (2) EIT. Experimental group: (1) EIT + L-NAC (5, 2, or 1 mM); (2) EIT + Mannitol (100, 50, or 10 mM); (3) EIT + Dex (20, 10, or 5 μg/mL); (4) EIT + L-NAC + Mannitol + Dex. After EIT was caused in anin-vitromodel of CI, explants were cultured in media containing L-NAC alone, Mannitol alone, or Dex alone at decreasing concentrations. Concentrations of L-NAC, Mannitol, and Dex that showed 50% protection of hair cell loss individually were used as a combination in experimental group 4.ResultsThere was an increase of total hair cell (THC) loss in the EIT OC explants when compared with control group HC counts or the tri-therapy cochlea. This study defined the dosage of L-NAC, Mannitol, and Dex for the survival of 50% protection of hair cellsin vitro. Their combination provided close to 96% protection, demonstrating an additive effect. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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