A Comprehensive Curation Shows the Dynamic Evolutionary Patterns of Prokaryotic CRISPRs.
Motivation. Clustered regularly interspaced short palindromic repeat (CRISPR) is a genetic element with active regulation roles for foreign invasive genes in the prokaryotic genomes and has been engineered to work with the CRISPR-associated sequence (Cas) gene Cas9 as one of the modern genome editin...
| Publicado en: | BioMed Research International Vol. 2016; pp. 1 - 8 |
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| Autores principales: | , , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
4/18/2016
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=114597640&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 114597640 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 4/18/2016 vid: 2016 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 114597640 114597640 114597640 10.1155/2016/7237053 114597640 ppf: 1 ppct: 7 formats: fmt: @attributes: type: P tig: atl: A Comprehensive Curation Shows the Dynamic Evolutionary Patterns of Prokaryotic CRISPRs. aug: au: Mai, Guoqin Ge, Ruiquan Sun, Guoquan Meng, Qinghan Zhou, Fengfeng affil: Shenzhen Institutes of Advanced Technology and Key Lab for Health Informatics, Chinese Academy of Sciences, Shenzhen, Guangdong 518055, China sug: subj: Evolution Genes Evaluation Genome Bacteria Classification Genes Classification Bacteria Analysis Genetic Techniques Funding Source Descriptive Statistics ab: Motivation. Clustered regularly interspaced short palindromic repeat (CRISPR) is a genetic element with active regulation roles for foreign invasive genes in the prokaryotic genomes and has been engineered to work with the CRISPR-associated sequence (Cas) gene Cas9 as one of the modern genome editing technologies. Due to inconsistent definitions, the existing CRISPR detection programs seem to have missed some weak CRISPR signals. Results. This study manually curates all the currently annotated CRISPR elements in the prokaryotic genomes and proposes 95 updates to the annotations. A new definition is proposed to cover all the CRISPRs. The comprehensive comparison of CRISPR numbers on the taxonomic levels of both domains and genus shows high variations for closely related species even in the same genus. The detailed investigation of how CRISPRs are evolutionarily manipulated in the 8 completely sequenced species in the genus Thermoanaerobacter demonstrates that transposons act as a frequent tool for splitting long CRISPRs into shorter ones along a long evolutionary history. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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