Enamelin Directs Crystallite Organization at the Enamel-Dentine Junction.
Enamel is an acellular material formed by the intricate process of amelogenesis. Disruption caused at the initial stages of development, by means of mutations in the ENAM gene encoding the enamelin protein, results in enamel hypoplasia. Little is known about the consequence of ENAM mutation on the e...
| Publicado en: | Journal of Dental Research Vol. 95; no. 5; pp. 580 - 588 |
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| Autores principales: | , |
| Formato: | research Journal Article |
| Publicado: |
Sage Publications Inc.
May2016
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=114697703&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 114697703 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00220345 1H7 jtl: Journal of Dental Research issn: 00220345 maglogo: Y pubinfo: dt: May2016 vid: 95 iid: 5 pid: 344 pub: Sage Publications Inc. place: Thousand Oaks, California artinfo: ui: 114697703 114697703 NLM26912218 114697703 10.1177/0022034516632745 NLM26912218 114697703 ppf: 580 ppct: 8 formats: tig: atl: Enamelin Directs Crystallite Organization at the Enamel-Dentine Junction. aug: au: Siddiqui, S. Al-Jawad, M. affil: Institute of Dentistry, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, UK sug: subj: Dental Enamel Proteins Dentin Crystallography Dentition X-Rays Methods Human Amelogenesis Imperfecta Case Control Studies Microscopy, Electron, Scanning Dental Enamel Hypoplasia Pathology Tooth, Deciduous Amelogenesis Imperfecta Pathology Validation Studies Comparative Studies Evaluation Research Multicenter Studies ab: Enamel is an acellular material formed by the intricate process of amelogenesis. Disruption caused at the initial stages of development, by means of mutations in the ENAM gene encoding the enamelin protein, results in enamel hypoplasia. Little is known about the consequence of ENAM mutation on the enamel structure at a crystallographic level. The aim of this study was to characterize the structure of ENAM-mutated enamel to develop a deeper understanding of the role of enamelin protein during formation with regard to crystal organization. Synchrotron X-ray microdiffraction (SXRD) and scanning electron microscopy (SEM) have been used to measure and correlate enamel crystallography and microstructure in hypoplastic and healthy enamel. Rietveld refinement carried out on 2-dimensional diffraction patterns, collected from the Advanced Photon Source, were used to quantify changes in the preferred orientation (crystallographic texture) within the labial regions of each tooth slice and then correlated with the local microstructure. In general, healthy deciduous incisors displayed a higher degree of crystal organization across the labial surface in comparison with the hypoplastic enamel. ENAM plays the greatest functional role at the enamel-dentine junction (EDJ), as it was the region that exhibited lowest texture relative to unaffected controls. Other areas within the tooth, however, such as the cusp tip, displayed greater organization in line with healthy enamel, suggesting its effects are restricted to the early stages of enamel secretion. Observed clinically, the surface of ENAM-mutated hypoplastic enamel can appear to be normal, yet severe sub-nano and microstructural defects appear beneath the subsurface layer. Quantitative characterization of the crystallographic properties from enamel with known genotype expands the understanding of enamel formation processes and can aid better clinical diagnosis and tailor-made treatment. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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