Urease inhibitory profile of extracts and chemical constituents of Pistacia atlantica ssp. cabulica Stocks.

The current study was designed to evaluate the urease inhibitory profile of extract and fractions ofPistacia atlanticassp. cabulica Stocks followed by bioactivity-guided isolated compounds. The crude extract was found significantly active with urease inhibitor (95.40% at 0.2 mg/mL) with IC50values o...

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Publicado en:Natural Product Research Vol. 30; no. 12; pp. 1411 - 1417
Autores principales: Uddin, Ghias, Ismail, Rauf, Abdur, Raza, Muslim, Khan, Haroon, Nasruddin, Khan, Majid, Farooq, Umar, Khan, Ajmal, Arifullah
Formato: Journal Article
Publicado: Taylor & Francis Ltd Jun2016
Acceso en línea:Ver este registro en EBSCOhost
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      dt: Jun2016
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      pub: Taylor & Francis Ltd
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        atl: Urease inhibitory profile of extracts and chemical constituents of Pistacia atlantica ssp. cabulica Stocks.
      aug:
        au:
          Uddin, Ghias
          Ismail
          Rauf, Abdur
          Raza, Muslim
          Khan, Haroon
          Nasruddin
          Khan, Majid
          Farooq, Umar
          Khan, Ajmal
          Arifullah
        affil: Institute of Chemical Sciences, Centre for Phytomedicine and Medicinal Organic Chemistry, University of Peshawar, Peshawar, Pakistan
      sug:
      ab: The current study was designed to evaluate the urease inhibitory profile of extract and fractions ofPistacia atlanticassp. cabulica Stocks followed by bioactivity-guided isolated compounds. The crude extract was found significantly active with urease inhibitor (95.40% at 0.2 mg/mL) with IC50values of 32.0 ± 0.28 μg/mL. Upon fractionation, ethyl acetate fraction displayed 100% urease inhibition with IC50values of 19.9 ± 0.51 μg/mL at 0.2 mg/mL. However,n-hexane and chloroform fractions exhibited insignificant urease inhibition. Similarly, the isolated compound, transilitin (1) and dihydro luteolin (2) demonstrated marked urease attenuation with 95 and 98% respectively, at 0.15 mg/mL. Both the isolated compounds showed marked potency with IC50values of 8.54 ± 0.54 and 9.58 ± 2.22 μg/mL, respectively. In short, both the extract and fractions and isolated compounds showed marked urease inhibition and thus a useful natural source of urease inhibition. Graphical abstract showing the imagePistacia atlantica, chemical structure of isolated compounds and urease enzyme.
      pubtype: Academic Journal
      doctype: Journal Article
      ougenre: Article
    language: English
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