Amlodipine Ameliorates Ischemia-Induced Neovascularization in Diabetic Rats through Endothelial Progenitor Cell Mobilization.
Objectives. We investigated whether amlodipine could improve angiogenic responses in a diabetic rat model of acute myocardial infarction (AMI) through improving bone marrow endothelial progenitor cell (EPC) mobilization, in the same way as angiotensin converting enzyme inhibitors. Methods. After ind...
| Publicado en: | BioMed Research International Vol. 2016; pp. 1 - 14 |
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| Autores principales: | , , , , , |
| Formato: | diagnostic images pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
5/8/2016
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=115191992&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 115191992 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 5/8/2016 vid: 2016 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 115191992 115191992 115191992 10.1155/2016/3182764 115191992 ppf: 1 ppct: 13 formats: fmt: @attributes: type: P tig: atl: Amlodipine Ameliorates Ischemia-Induced Neovascularization in Diabetic Rats through Endothelial Progenitor Cell Mobilization. aug: au: Sun, Jiayin Xie, Jun Kang, Lina Ferro, Albert Dong, Li Xu, Biao affil: Department of Cardiology, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China sug: subj: Amlodipine Administration and Dosage Neovascularization, Pathologic Drug Therapy Diabetes Mellitus Complications Stem Cells Cell Movement Endothelium Myocardial Infarction Complications Neovascularization, Physiologic Drug Effects Signal Transduction Drug Effects Animal Studies Rats Models, Biological Random Assignment Perindopril Administration and Dosage Pretest-Posttest Design Heart Physiology Ventricular Remodeling Drug Effects Fibrosis Apoptosis Drug Effects Vascular Endothelial Growth Factors Drug Effects Oxidoreductases Drug Effects Protein Kinases Drug Effects Echocardiography Biological Assay Blotting, Western Descriptive Statistics T-Tests One-Way Analysis of Variance Post Hoc Analysis Data Analysis Software P-Value Flow Cytometry Reverse Transcriptase Polymerase Chain Reaction Funding Source ab: Objectives. We investigated whether amlodipine could improve angiogenic responses in a diabetic rat model of acute myocardial infarction (AMI) through improving bone marrow endothelial progenitor cell (EPC) mobilization, in the same way as angiotensin converting enzyme inhibitors. Methods. After induction of AMI by coronary artery ligation, diabetic rats were randomly assigned to receive perindopril (2 mgkg−1 day−1), amlodipine (2.5 mgkg−1 day−1), or vehicle by gavage (n=20 per group). Circulating EPC counts before ligation and on days 1, 3, 5, 7, 14, and 28 after AMI were measured in each group. Microvessel density, cardiac function, and cardiac remodeling were assessed 4 weeks after treatment. The signaling pathway related to EPC mobilization was also measured. Results. Circulating EPC count in amlodipine- and perindopril-treated rats peaked at day 7, to an obvious higher level than the control group peak which was reached earlier (at day 5). Rats treated with amlodipine showed improved postischemia neovascularization and cardiac function, together with reduced cardiac remodeling, decreased interstitial fibrosis, and cardiomyocyte apoptosis. Amlodipine treatment also increased cardiac SDF-1/CXCR4 expression and gave rise to activation of VEGF/Akt/eNOS signaling in bone marrow. Conclusions. Amlodipine promotes neovascularization by improving EPC mobilization from bone marrow in diabetic rats after AMI, and activation of VEGF/Akt/eNOS signaling may in part contribute to this. pubtype: Academic Journal doctype: diagnostic images pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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