Simultaneous visualization of tumour oxygenation, neovascularization and contrast agent perfusion by real-time three-dimensional optoacoustic tomography.
Objectives: Intravital imaging within heterogenic solid tumours is important for understanding blood perfusion profiles responsible for establishment of multiple parameters within the tumour mass, such as hypoxic and nutrition gradients, cell viability, proliferation and drug response potentials.Met...
| Publicado en: | European Radiology Vol. 26; no. 6; pp. 1843 - 1852 |
|---|---|
| Autores principales: | , , , , , |
| Formato: | diagnostic images pictorial research tables/charts Journal Article |
| Publicado: |
Springer Nature
Jun2016
|
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=115269348&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 115269348 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 09387994 NPH jtl: European Radiology issn: 09387994 maglogo: N pubinfo: dt: Jun2016 vid: 26 iid: 6 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 115269348 115269348 NLM26334513 115269348 10.1007/s00330-015-3980-0 NLM26334513 115269348 ppf: 1843 ppct: 9 formats: fmt: @attributes: type: P tig: atl: Simultaneous visualization of tumour oxygenation, neovascularization and contrast agent perfusion by real-time three-dimensional optoacoustic tomography. aug: au: Ermolayev, Vladimir Dean-Ben, Xose Mandal, Subhamoy Ntziachristos, Vasilis Razansky, Daniel Dean-Ben, Xose Luis affil: Institute for Biological and Medical Imaging (IBMI), Helmholtz Center Munich, Ingolstädter Landstraβe 1 85764 Neuherberg Germany sug: subj: Neovascularization, Pathologic Acoustics Methods Animal Studies Mice Indoles Pharmacokinetics Imaging, Three-Dimensional Methods Oxygen Consumption Perfusion Cross Sectional Studies Tomography Methods Female Contrast Media Pharmacokinetics Female ab: Objectives: Intravital imaging within heterogenic solid tumours is important for understanding blood perfusion profiles responsible for establishment of multiple parameters within the tumour mass, such as hypoxic and nutrition gradients, cell viability, proliferation and drug response potentials.Methods: Herein, we developed a method based on a volumetric multispectral optoacoustic tomography (vMSOT) for cancer imaging in preclinical models and explored its capacity for three-dimensional imaging of anatomic, vascular and functional tumour profiles in real time.Results: In contrast to methods based on cross-sectional (2D) image acquisition as a basis for 3D rendering, vMSOT has attained concurrent observations from the entire tumour volume at 10 volumetric frames per second. This truly four dimensional imaging performance has enabled here the simultaneous assessment of blood oxygenation gradients and vascularization in solid breast tumours and revealed different types of blood perfusion profiles in-vivo.Conclusion: The newly introduced capacity for high-resolution three-dimensional tracking of fast tumour perfusion suggests vMSOT as a powerful method in preclinical cancer research and theranostics. As the imaging setup can be equally operated in both stationary and handheld mode, the solution is readily translatable for perfusion monitoring in a clinical setting.Key Points: • vMSOT visualizes 3D anatomic, vascular and functional tumour profiles in real time. • Three types of blood perfusion profiles are revealed in breast tumour model. • The method is readily adaptable to operate in a handheld clinical mode. pubtype: Academic Journal doctype: diagnostic images pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|