Cryptococcus tetragattii as a major cause of cryptococcal meningitis among HIV-infected individuals in Harare, Zimbabwe.

Objectives: HIV-associated cryptococcal meningitis is commonly caused by Cryptococcus neoformans, whilst infections with Cryptococcus gattii sensu lato are historically rare. Despite available studies, little is known about the occurrence of C. gattii sensu lato infections among HIV-infected individ...

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Publicado en:Journal of Infection Vol. 72; no. 6; pp. 745 - 753
Autores principales: Nyazika, Tinashe K., Hagen, Ferry, Meis, Jacques F., Robertson, Valerie J.
Formato: research Journal Article
Publicado: W B Saunders Jun2016
Acceso en línea:Ver este registro en EBSCOhost
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      dt: Jun2016
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      pub: W B Saunders
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        10.1016/j.jinf.2016.02.018
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        atl: Cryptococcus tetragattii as a major cause of cryptococcal meningitis among HIV-infected individuals in Harare, Zimbabwe.
      aug:
        au:
          Nyazika, Tinashe K.
          Hagen, Ferry
          Meis, Jacques F.
          Robertson, Valerie J.
        affil: Department of Chemical Pathology, College of Health Sciences, University of Zimbabwe, Harare, Zimbabwe
      sug:
        subj:
          Meningitis, Cryptococcal Microbiology
          Cryptococcus
          AIDS-Related Opportunistic Infections Microbiology
          Sequence Analysis
          Mycological Typing Techniques
          Female
          DNA
          Hospital Mortality
          Prevalence
          Male
          Cryptococcosis Epidemiology
          Cryptococcosis Microbiology
          Cerebrospinal Fluid Microbiology
          Prospective Studies
          Meningitis, Cryptococcal Epidemiology
          Genotype
          Middle Age
          Adult
          Zimbabwe
          Funding Source
          Human
          Middle Aged: 45-64 years
          Adult: 19-44 years
          Female
          Male
      ab: Objectives: HIV-associated cryptococcal meningitis is commonly caused by Cryptococcus neoformans, whilst infections with Cryptococcus gattii sensu lato are historically rare. Despite available studies, little is known about the occurrence of C. gattii sensu lato infections among HIV-infected individuals in Zimbabwe.Methods: In a prospective cohort, we investigated the prevalence of C. gattii sensu lato meningitis among HIV-infected patients (n = 74) in Harare, Zimbabwe.Results: Of the 66/74 isolates confirmed by molecular characterization, 16.7% (11/66) were found to be C. gattii sensu lato and 83.3% (55/66) C. neoformans sensu stricto. From one patient two phenotypically different C. gattii sensu lato colonies were cultured. The majority (n = 9/12; 75%) of the C. gattii sensu lato isolates were Cryptococcus tetragattii (AFLP7/VGIV), which has been an infrequently reported pathogen. In-hospital mortality associated with C. gattii sensu lato was 36.4%.Conclusions: Our data suggests that C. tetragattii (AFLP7/VGIV) is a more common cause of disease than C. gattii sensu stricto (genotype AFLP4/VGI) among patients with HIV-associated cryptococcal meningitis in Harare, Zimbabwe and possibly underreported in sub-Saharan Africa.
      pubtype: Academic Journal
      doctype:
        research
        Journal Article
      ougenre: Article
    language: English
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