Human serum preβ1-high density lipoprotein levels are independently and negatively associated with coronary artery diseases.
Background: Serum preβ1-high density lipoprotein (preβ1-HDL) was defined by two-dimensional non-denaturing linear gel electrophoresis and apolipoprotein A-I immuno-blotting. Serum preβ1-HDL seems to play an important role in reverse cholesterol transport, a well-known anti-atherosclerosis process. H...
| Publicado en: | Nutrition & Metabolism Vol. 13; pp. 1 - 13 |
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| Autores principales: | , , , , , , , , , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
BioMed Central
5/17/2016
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=115441570&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 115441570 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 17437075 1CYX jtl: Nutrition & Metabolism issn: 17437075 maglogo: N pubinfo: dt: 5/17/2016 vid: 13 pid: 24147 pub: BioMed Central artinfo: ui: 115441570 115441570 115441570 10.1186/s12986-016-0093-y 115441570 ppf: 1 ppct: 12 formats: fmt: @attributes: type: P tig: atl: Human serum preβ1-high density lipoprotein levels are independently and negatively associated with coronary artery diseases. aug: au: Yunqin Chen Jibin Dong Xueying Chen Hui Jiang Bakillah, Ahmed Xiaojin Zhang Zhiqiang Li Jia Yin Donghui Liang Yunzeng Zou Hussain, Mahmood Cuchel, Marina Rader, Daniel Haozhu Chen Junbo Ge Xian-Cheng Jiang affil: Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai, China sug: subj: Coronary Arteriosclerosis Lipoproteins, HDL Blood Lipoproteins, HDL Immunology Lipoproteins, HDL Analysis Chemistry, Analytical Methods Electrophoresis, Agar Gel Methods Immunoblotting Methods Human Microscopy, Electron Descriptive Statistics Data Analysis Software Academic Medical Centers China Analysis of Variance Linear Regression Logistic Regression Female Male Middle Age Adult Aged Funding Source Middle Aged: 45-64 years Adult: 19-44 years Aged: 65+ years Female Male ab: Background: Serum preβ1-high density lipoprotein (preβ1-HDL) was defined by two-dimensional non-denaturing linear gel electrophoresis and apolipoprotein A-I immuno-blotting. Serum preβ1-HDL seems to play an important role in reverse cholesterol transport, a well-known anti-atherosclerosis process. However, there are still debatable questions for its quantification and coronary artery disease (CAD) relevance. Methods: We isolated the preβ1-HDL using a new native polyacrylamide gel electrophoresis (PAGE) system and lipid pre-staining serum. We established a two-demensional gel electrophoresis system. Results: We measured the preβ1-HDL in Tangier disease patients and subjects with cholesterol ester transfer protein (CETP) mutation. The preβ1-HDL is clearly separated from lipid-free apoA-I monomer and cannot be converted into other HDL particles under lecithin-cholesterol acyltransferase (LCAT) inhibition. This preβ1-HDL is a spheroidal particle with the highest apoA-1/cholesterol ratio and highest density (=1.21 g/ml), as compared with all other HDLs. Importantly, we found that serum from subjects with Tangier disease or with cholesterol ester transfer protein (CETP) mutation have no detectible preβ1-HDL particles. We recruited a total of 102 subjects underwent diagnostic coronary angiography and measured their preβ1-HDL levels. Among them, 56 had no stenosis of coronary artery and 46 were diagnosed as CAD, which was predefined as the presence of a luminal diameter stenosis =50 % in at least 1 major coronary artery territory. We found that preβ1-HDL is independently and negatively associated with the severity of the coronary artery stenosis (Gensini score). Conclusion: We established a novel and simple method for human serum preβ1-HDL quantification. We found β pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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