Early Gut Colonization of Preterm Infants: Effect of Enteral Feeding Tubes.
Objective: The aim of the study was to evaluate the potential colonization of nosocomial bacteria in enteral feeding systems and its effect on early gut colonization of preterm neonates.Methods: Mother's own milk, donor milk, and preterm formula samples obtained after passing through the external pa...
| Published in: | Journal of Pediatric Gastroenterology & Nutrition Vol. 62; no. 6; pp. 893 - 901 |
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| Main Authors: | , , , , , , , |
| Format: | pictorial research tables/charts Journal Article |
| Published: |
Wiley-Blackwell
Jun2016
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=115592279&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 115592279 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 02772116 N1F jtl: Journal of Pediatric Gastroenterology & Nutrition issn: 02772116 maglogo: N pubinfo: dt: Jun2016 vid: 62 iid: 6 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 115592279 115592279 NLM26741949 115592279 10.1097/MPG.0000000000001104 NLM26741949 115592279 ppf: 893 ppct: 8 formats: tig: atl: Early Gut Colonization of Preterm Infants: Effect of Enteral Feeding Tubes. aug: au: Gómez, Marta Moles, Laura Melgar, Ana Ureta, Noelia Bustos, Gerardo Fernández, Leónides Rodríguez, Juan M. Jiménez, Esther affil: Departamento Nutrición, Bromatología y Tecnología de los Alimentos, Universidad Complutense de Madrid, Madrid, Spain sug: subj: Cross Infection Diagnosis Intestines Microbiology Enteral Nutrition Adverse Effects Infant, Newborn Infant, Premature Male Feces Microbiology Female Meconium Microbiology Infant Human Infant, Newborn: birth-1 month Infant: 1-23 months Male Female ab: Objective: The aim of the study was to evaluate the potential colonization of nosocomial bacteria in enteral feeding systems and its effect on early gut colonization of preterm neonates.Methods: Mother's own milk, donor milk, and preterm formula samples obtained after passing through the external part of the enteral feeding tubes were cultured. In addition, meconium and fecal samples from 26 preterm infants collected at different time points until discharge were cultured. Random amplification polymorphism DNA and pulse field gel electrophoresis were performed to confirm the presence of specific bacterial strains in milk and infant fecal samples.Results: Approximately 4000 bacterial isolates were identified at the species level. The dominant species in both feces from preterm infants and milk samples were Staphylococcus epidermidis, S aureus, Enterococcus faecalis, E faecium, Serratia marcescens, Klebsiella pneumoniae, and Escherichia coli. All of them were present at high concentrations independently of the feeding mode. Random amplification polymorphism DNA and pulse field gel electrophoresis techniques showed that several bacteria strains were found in both type of samples. Furthermore, scanning electron microscopy revealed the presence of a dense bacterial biofilm in several parts of the feeding tubes and the tube connectors.Conclusions: There is a sharing of bacterial strains between the neonates' gastrointestinal microbiota and the feeding tubes used to feed them. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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