Involvement of the Nonneuronal Cholinergic System in Bone Remodeling in Rat Midpalatal Suture after Rapid Maxillary Expansion.
Few studies sought to analyze the expression and function of the nonneuronal acetylcholine system in bone remodeling in vivo due to the lack of suitable models. We established a rat maxilla expansion model in which the midline palatine suture of the rat was rapidly expanded under mechanical force ap...
| Published in: | BioMed Research International Vol. 2016; pp. 1 - 9 |
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| Main Authors: | , , , , , , |
| Format: | research tables/charts Journal Article |
| Published: |
Wiley-Blackwell
7/10/2016
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=116699680&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 116699680 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 7/10/2016 vid: 2016 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 116699680 116699680 116699680 10.1155/2016/8106067 116699680 ppf: 1 ppct: 8 formats: fmt: @attributes: type: P tig: atl: Involvement of the Nonneuronal Cholinergic System in Bone Remodeling in Rat Midpalatal Suture after Rapid Maxillary Expansion. aug: au: Che, Xiaoxia Guo, Jie Wang, Lue Miao, Cong Ge, Lihua Tian, Zhenchuan Wang, Jianhong affil: Department of Orthodontics, School of Stomatology, Capital Medical University, Beijing 100050, China sug: subj: Bone Remodeling Acetylcholine Palate Maxilla Surgery Models, Biological Animal Studies Rats In Vivo Studies RNA Analysis Receptors, Cell Surface Membrane Proteins Immunohistochemistry Polymerase Chain Reaction Methods Bone Resorption Data Analysis Software Descriptive Statistics One-Way Analysis of Variance Kruskal-Wallis Test P-Value Funding Source Orthodontics, Corrective ab: Few studies sought to analyze the expression and function of the nonneuronal acetylcholine system in bone remodeling in vivo due to the lack of suitable models. We established a rat maxilla expansion model in which the midline palatine suture of the rat was rapidly expanded under mechanical force application, inducing tissue remodeling and new bone formation, which could be a suitable model to investigate the role of the nonneuronal acetylcholine system in bone remodeling in vivo. During the expansion, the expression pattern changes of the nonneuronal cholinergic system components and the mRNA levels of OPG/RANKL were detected by immunohistochemistry or real-time PCR. The value of the RANKL/OPG ratio significantly increased after 1 day of expansion, indicating dominant bone resorption induced by the mechanical stimulation; however after 3 days of expansion, the value of the RANKL/OPG ratio significantly decreased, suggesting a dominant role of the subsequent bone formation process. Increasing expression of Ach was detected after 3 days of expansion which indicated that ACh might play a role in bone formation. The mRNA expression levels of other components also showed observable changes during the expansion which confirmed the involvement of the nonneuronal cholinergic system in the process of bone remodeling in vivo. Further researches are still needed to figure out the detailed functions of the nonneuronal cholinergic system and its components. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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