Monodisperse Uni- and Multicompartment Liposomes.
Liposomes are self-assembled phospholipid vesicles with great potential in fields ranging from targeted drug delivery to artificial cells. The formation of liposomes using microfluidic techniques has seen considerable progress, but the liposomes formation process itself has not been studied in great...
| Publicado en: | Journal of the American Chemical Society Vol. 138; no. 24; pp. 7584 - 7592 |
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| Autores principales: | , , |
| Formato: | Artículo |
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American Chemical Society
6/22/2016
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| Materias: | |
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=hlh&AN=116707312&site=ehost-live header: @attributes: shortDbName: hlh uiTerm: 116707312 longDbName: Humanities International Complete uiTag: AN controlInfo: bkinfo: jinfo: jid: 00027863 ACS jtl: Journal of the American Chemical Society issn: 00027863 maglogo: N pubinfo: dt: 6/22/2016 vid: 138 iid: 24 pid: 997 pub: American Chemical Society artinfo: ui: 116707312 10.1021/jacs.6b02107 ppf: 7584 ppct: 8 formats: tig: atl: Monodisperse Uni- and Multicompartment Liposomes. aug: au: Nan-Nan Deng Yelleswarapu, Maaruthy Huck, Wilhelm T. S. affil: Radboud University, Institute for Molecules and Materials, Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands su: Liposomes Cytoplasm Phospholipids Lipofection Chemical reactions Reactivity (Chemistry) Chemical processes Click chemistry sug: subj: Liposomes Cytoplasm Phospholipids Lipofection Chemical reactions Reactivity (Chemistry) Chemical processes Click chemistry ab: Liposomes are self-assembled phospholipid vesicles with great potential in fields ranging from targeted drug delivery to artificial cells. The formation of liposomes using microfluidic techniques has seen considerable progress, but the liposomes formation process itself has not been studied in great detail. As a result, high throughput, high-yielding routes to monodisperse liposomes with multiple compartments have not been demonstrated. Here, we report on a surfactant-assisted microfluidic route to uniform, single bilayer liposomes, ranging from 25 to 190 μm, and with or without multiple inner compartments. The key of our method is the precise control over the developing interfacial energies of complex W/O/W emulsion systems during liposome formation, which is achieved via an additional surfactant in the outer water phase. The liposomes consist of single bilayers, as demonstrated by nanopore formation experiments and confocal fluorescence microscopy, and they can act as compartments for cell-free gene expression. The microfluidic technique can be expanded to create liposomes with a multitude of coupled compartments, opening routes to networks of multistep microreactors. pubtype: Academic Journal doctype: Article src: R language: English refInfo: copyright: @attributes: flag: Y dt: @attributes: year: 2016 holdings: @attributes: islocal: N |
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