Beneficial Effect of Short Pretransplant Period of Hypothermic Pulsatile Perfusion of the Warm-Ischemic Kidney after Cold Storage: Experimental Study.
Warm ischemia (WI) produces a significant deleterious effect in potential kidney grafts. Hypothermic machine perfusion (HMP) seems to improve immediate graft function after transplant. Our aim was to analyze the effect of short pretransplant periods of pulsatile HMP on histology and renal injury in...
| Published in: | BioMed Research International Vol. 2016; pp. 1 - 9 |
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| Main Authors: | , , , , , , , |
| Format: | pictorial research tables/charts Journal Article |
| Published: |
Wiley-Blackwell
7/31/2016
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=117105029&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 117105029 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 7/31/2016 vid: 2016 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 117105029 117105029 117105029 10.1155/2016/2518626 117105029 ppf: 1 ppct: 8 formats: fmt: @attributes: type: P tig: atl: Beneficial Effect of Short Pretransplant Period of Hypothermic Pulsatile Perfusion of the Warm-Ischemic Kidney after Cold Storage: Experimental Study. aug: au: Lázaro, Alberto Humanes, Blanca Jado, Juan Carlos Mojena, Marina González-Nicolás, María Ángeles del Cañizo, Juan Francisco Tejedor, Alberto Lledó-García, Enrique affil: Renal Physiopathology Laboratory, Department of Nephrology, Instituto de Investigación Sanitaria Gregorio Marañón (IiSGM), Hospital General Universitario Gregorio Marañón, 28007 Madrid, Spain sug: subj: Kidney Transplantation Methods Ischemia Organ Preservation Methods Perfusion Hypothermia, Induced Cold Heat Experimental Studies Animal Studies Swine Nephrectomy Kidney Analysis Inflammation Fibrosis Apoptosis Tissue Culture Techniques Immunohistochemistry Microscopy Collagen Staining and Labeling P-Value Descriptive Statistics Post Hoc Analysis Kruskal-Wallis Test Data Analysis Software Kidney Injuries Transforming Growth Factor beta Funding Source ab: Warm ischemia (WI) produces a significant deleterious effect in potential kidney grafts. Hypothermic machine perfusion (HMP) seems to improve immediate graft function after transplant. Our aim was to analyze the effect of short pretransplant periods of pulsatile HMP on histology and renal injury in warm-ischemic kidneys. Twelve minipigs were used. WI was achieved in the right kidney by applying a vascular clamp for 45 min. After nephrectomy, autotransplant was performed following one of two strategies: cold storage of the kidneys or cold storage combined with perfusion in pulsatile HMP. The graft was removed early to study renal morphology, inflammation (fibrosis), and apoptosis. Proinflammatory activity and fibrosis were less pronounced after cold storage of the kidneys with HMP than after cold storage only. The use of HMP also decreased apoptosis compared with cold storage only. The detrimental effects on cells of an initial and prolonged period of WI seem to improve with a preservation protocol that includes a short period of pulsatile HMP after cold storage and immediately before the transplant, in comparison with cold storage only. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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