Automated Extraction of Formalin-Fixed, Paraffin-Embedded Tissue for High-Risk Human Papillomavirus Testing of Head and Neck Squamous Cell Carcinomas Using the Roche Cobas 4800 System.
Context.--Testing for high-risk human papillomavirus (HR-HPV) in head and neck squamous cell carcinomas (HNSCCs) is important for both prognostication and clinical management. Several testing platforms are available for HR-HPV; however, effective alternative automated approaches are needed. Objectiv...
| Published in: | Archives of Pathology & Laboratory Medicine Vol. 140; no. 8; pp. 844 - 849 |
|---|---|
| Main Authors: | , , , , , , |
| Format: | research tables/charts Journal Article |
| Published: |
College of American Pathologists
Aug2016
|
| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=117119108&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 117119108 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00039985 1FS jtl: Archives of Pathology & Laboratory Medicine issn: 00039985 maglogo: N pubinfo: dt: Aug2016 vid: 140 iid: 8 pid: 2550 pub: College of American Pathologists place: Northfield, Illinois artinfo: ui: 117119108 117119108 117119108 10.5858/arpa.2015-0272-OA 117119108 ppf: 844 ppct: 5 formats: fmt: @attributes: type: P tig: atl: Automated Extraction of Formalin-Fixed, Paraffin-Embedded Tissue for High-Risk Human Papillomavirus Testing of Head and Neck Squamous Cell Carcinomas Using the Roche Cobas 4800 System. aug: au: Kerr, Darcy A. Sweeney, Brenda Arpin III, Ronald N. Ring, Melissa Pitman, Martha B. Wilbur, David C. Faquin, William C. affil: Department of Pathology, Massachusetts General Hospital, Boston sug: subj: Head and Neck Neoplasms Diagnosis Head and Neck Neoplasms Microbiology Autoanalyzers Evaluation Carcinoma, Squamous Cell Diagnosis Equipment Reliability Papillomaviruses Analysis Prognosis Polymerase Chain Reaction Immunohistochemistry In Situ Hybridization Paraffin Embedding Specimen Handling Evaluation Research Sensitivity and Specificity Retrospective Design Predictive Value of Tests Descriptive Statistics Human ab: Context.--Testing for high-risk human papillomavirus (HR-HPV) in head and neck squamous cell carcinomas (HNSCCs) is important for both prognostication and clinical management. Several testing platforms are available for HR-HPV; however, effective alternative automated approaches are needed. Objective.--To assess the performance of the automated Roche cobas 4800 HPV real-time polymerase chain reaction-based system on formalin-fixed, paraffin-embedded HNSCC specimens and compare results with standard methods of in situ hybridization (ISH) and p16 immuno-histochemistry. Design.--Formalin-fixed, paraffin-embedded samples of HNSCC were collected from archival specimens in the Department of Pathology, Massachusetts General Hospital (Boston), and prepared using the automated system by deparaffinization and dehydration followed by tissue lysis. Samples were integrated into routine cervical cytology testing runs by cobas. Corresponding formalin-fixed, paraffin-embedded samples were evaluated for HR-HPV by ISH and p16 by immunohistochemistry. Discrepant cases were adjudicated by polymerase chain reaction. Results.--Sixty-two HNSCC samples were analyzed using the automated cobas system, ISH, and immunohistochemistry. Fifty-two percent (n = 32 of 62) of formalin-fixed, paraffin-embedded tumors were positive for HR-HPV by cobas. Eighty-eight percent (n = 28 of 32) of cases were the HPV 16 subtype and 12% (n = 4 of 32) were other HR-HPV subtypes. Corresponding testing with ISH was concordant in 92% (n = 57 of 62) of cases. Compared with the adjudication polymerase chain reaction standard, there were 3 false-positive cases by cobas. Conclusions.--Concordance in HNSCC HR-HPV status between cobas and ISH was more than 90%. The cobas demonstrated a sensitivity of 100% and a specificity of 91 % for detection of HR-HPV. Advantages favoring cobas include its automation, cost efficiency, objective results, and ease of performance. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|