Effects of diagnostic ultrasound-targeted microbubble destruction on the homing ability of bone marrow stromal cells to the kidney parenchyma.
Objectives: Bone marrow stromal cells (BMSC) transplantation proves successful in treating kidney disease and injury in many studies. However, their reparative capacity is limited by the poor homing ability in vivo, which is decided mainly by the local expression of chemoattractants. Our study explo...
| Publicado en: | European Radiology Vol. 26; no. 9; pp. 3006 - 3017 |
|---|---|
| Autores principales: | , , , , , , , , , |
| Formato: | diagnostic images pictorial research tables/charts Journal Article |
| Publicado: |
Springer Nature
Sep2016
|
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=117171339&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 117171339 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 09387994 NPH jtl: European Radiology issn: 09387994 maglogo: N pubinfo: dt: Sep2016 vid: 26 iid: 9 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 117171339 117171339 NLM26637447 117171339 10.1007/s00330-015-4123-3 NLM26637447 117171339 ppf: 3006 ppct: 11 formats: fmt: @attributes: type: P tig: atl: Effects of diagnostic ultrasound-targeted microbubble destruction on the homing ability of bone marrow stromal cells to the kidney parenchyma. aug: au: Wang, Gong Zhang, Qian Zhuo, Zhongxiong Wu, Shengzheng Liu, Zheng Xia, Hongmei Tan, Kaibin Zou, Linru Gan, Ling Gao, Yunhua affil: Department of Ultrasound , Xinqiao Hospital, The Third Military Medical University , Chongqing 400037 China sug: subj: Connective Tissue Cells Physiology Ultrasonography Methods Bone Marrow Transplantation Methods Kidney Physiology Equipment and Supplies Rats Microscopy Blotting, Western Animal Studies Enzyme-Linked Immunosorbent Assay ab: Objectives: Bone marrow stromal cells (BMSC) transplantation proves successful in treating kidney disease and injury in many studies. However, their reparative capacity is limited by the poor homing ability in vivo, which is decided mainly by the local expression of chemoattractants. Our study explored the mechanical effects of ultrasound targeted microbubble destruction (UTMD) on BMSCs homing ability in treated kidney tissues.Methods: Rats were injected with red fluorescent protein (RFP)-labelled BMSCs and sonicated with microbubble-mediated ultrasound. Then, we tested kidney micro-environment changes induced and their influence on stem cell homing ability.Results: The results showed that the mechanical effects of UTMD would increase local and transient levels of chemoattractants (i.e. cytokines, integrins and growth factors) in targeted kidney tissues. Transmission electron microscopy showed that vascular endothelial cell was discontinuous in the UTMD group post-treatment, becoming smooth 72 h later. Confocal laser scanning microscopy and RT-PCR showed up to eight times more stem cells in the peritubular regions of experimental kidneys on days 1 and 3 post-treatment compared with the contralateral kidney.Conclusions: These results confirmed that renal micro-environment changes caused by appropriate UTMD may promote BMSC homing ability toward treated kidney tissues without renal toxicity and cell damage.Key Points: • This experiment showed a feasible strategy in promoting stem cell homing ability. • The treatment uses diagnostic ultrasound during enhancement with IV microbubbles. • A suitable micro-environment was important for targeted stem cell homing and retention. • The method is effective for stem cell homing to kidney diseases. • More work is required with larger animals before potential human trials. pubtype: Academic Journal doctype: diagnostic images pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|