Role of miRNAs in Epicardial Adipose Tissue in CAD Patients with T2DM.
Background. Epicardial adipose tissue (EAT) is identified as an atypical fat depot surrounding the heart with a putative role in the involvement of metabolic disorders, including obesity, type-2 diabetes mellitus, and atherosclerosis. We profiled miRNAs in EAT of metabolic patients with coronary art...
| Publicado en: | BioMed Research International Vol. 2016; pp. 1 - 8 |
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| Autores principales: | , , , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
8/14/2016
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=117447428&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 117447428 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 8/14/2016 vid: 2016 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 117447428 117447428 117447428 10.1155/2016/1629236 117447428 ppf: 1 ppct: 7 formats: fmt: @attributes: type: P tig: atl: Role of miRNAs in Epicardial Adipose Tissue in CAD Patients with T2DM. aug: au: Liu, Yang Fu, Wenbo Lu, Mu Huai, Shitao Song, Yaqin Wei, Yutao affil: Medicine Department, Shihezi University, Shihezi, Xinjiang 832000, China sug: subj: Diabetes Mellitus, Type 2 Pathology Coronary Arteriosclerosis Pathology RNA Analysis Pericardium Adipose Tissue RNA Classification Human Microarray Analysis China P-Value Descriptive Statistics Bioinformatics Data Analysis Software Male Female Academic Medical Centers Signal Transduction Aged Middle Age Chi Square Test Unpaired T-Tests Wilcoxon Signed Rank Test Funding Source Aged: 65+ years Middle Aged: 45-64 years Male Female ab: Background. Epicardial adipose tissue (EAT) is identified as an atypical fat depot surrounding the heart with a putative role in the involvement of metabolic disorders, including obesity, type-2 diabetes mellitus, and atherosclerosis. We profiled miRNAs in EAT of metabolic patients with coronary artery disease (CAD) and type-2 diabetes mellitus (T2DM) versus metabolically healthy patients by microarray. Compared to metabolically healthy patients, we identified forty-two miRNAs that are differentially expressed in patients with CAD and T2DM from Xinjiang, China. Eleven miRNAs were selected as potential novel miRNAs according to P value and fold change. Then the potential novel miRNAs targeted genes were predicted via TargetScan, PicTar, and miRTarbase, and the function of the target genes was predicted via Gene Ontology (GO) analysis while the enriched KEGG pathway analyses of the miRNAs targeted genes were performed by bioinformatics software DAVID. Then protein-protein interaction networks of the targeted gene were conducted by online software STRING. Finally, using microarray, bioinformatics approaches revealed the possible molecular mechanisms pathogenesis of CAD and T2DM. A total of 11 differentially expressed miRNAs were identified and among them, hsa-miR-4687-3p drew specific attention. Bioinformatics analysis revealed that insulin signaling pathway is the central way involved in the progression of metabolic disorders. Conclusions. The current findings support the fact that miRNAs are involved in the pathogenesis of metabolic disorders in EAT of CAD patients with T2DM, and validation of the results of these miRNAs by independent and prospective study is certainly warranted. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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