Imaging Early Fate of Cancer Stem Cells in Mouse Hindlimbs with Sodium Iodide Symporter Gene and I-124 PET.
Purpose: We investigated the capacity of sodium/iodide symporter (NIS) positron emission tomography (PET) to image and quantitate early engraftment and survival of cancer stem cells (CSCs) in living mice.Procedures: CT26 colon cancer cells and CSCs were infected with an adenovirus expressing both NI...
| Publicado en: | Molecular Imaging & Biology Vol. 18; no. 5; pp. 748 - 758 |
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| Autores principales: | , , , , , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Springer Nature
Oct2016
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=117881399&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 117881399 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 15361632 KJU jtl: Molecular Imaging & Biology issn: 15361632 maglogo: N pubinfo: dt: Oct2016 vid: 18 iid: 5 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 117881399 117881399 NLM26914278 117881399 10.1007/s11307-016-0941-1 NLM26914278 117881399 ppf: 748 ppct: 10 formats: fmt: @attributes: type: P tig: atl: Imaging Early Fate of Cancer Stem Cells in Mouse Hindlimbs with Sodium Iodide Symporter Gene and I-124 PET. aug: au: Park, Jin Jung, Kyung-Ho Lee, Jin Moon, Seung Cho, Young Choe, Yearn Lee, Kyung-Han Park, Jin Won Lee, Jin Hee Moon, Seung Hwan Cho, Young Seok Choe, Yearn Seung affil: Department of Nuclear Medicine, Samsung Medical Center , Sungkyunkwan University School of Medicine , Seoul 06351 Korea sug: subj: Sodium-Iodide Symporters Metabolism Stem Cells Pathology Stem Cells Metabolism Lower Extremity Tomography, Emission-Computed Methods Microscopy Cells Metabolism Viruses Metabolism Iodine Radioisotopes Genetics Animal Studies Cells Pathology Proteins Metabolism Mice Reproducibility of Results Diagnostic Imaging Cell Line, Tumor ab: Purpose: We investigated the capacity of sodium/iodide symporter (NIS) positron emission tomography (PET) to image and quantitate early engraftment and survival of cancer stem cells (CSCs) in living mice.Procedures: CT26 colon cancer cells and CSCs were infected with an adenovirus expressing both NIS and enhanced green fluorescent protein (EGFP). Cells were implanted into normal and ischemic hindlimbs of mice, and serial optical and I-124 PET imaging was performed. Extracted tissues underwent I-124 measurements and confocal microscopy.Results: NIS.EGFP gene transfer increased fluorescence and I-124 uptake of CSCs and CT26 cells without adverse effects. I-124 PET clearly visualized implanted tumor cells in vivo, whereas optical imaging was suboptimal. PET revealed 1.95, 2.22, and 1.93-fold greater I-124 uptake by CSC inoculation into ischemic compared to non-ischemic limbs at 2, 15, and 24 h, respectively. CT26 cells showed similar but smaller differences. PET findings were confirmed by ex vivo measurements and confocal microscopy.Conclusions: NIS PET can help identify microenvironment conditions that influence early survival of implanted CSCs. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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