Detection of Lymph Node Metastases with SERRS Nanoparticles.
Purpose: The accurate detection of lymph node metastases in prostate cancer patients is important to direct treatment decisions. Our goal was to develop an intraoperative imaging approach to distinguish normal from metastasized lymph nodes. We aimed at developing and testing gold-silica surface-enha...
| Publicado en: | Molecular Imaging & Biology Vol. 18; no. 5; pp. 677 - 686 |
|---|---|
| Autores principales: | , , , , , , , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Springer Nature
Oct2016
|
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=117881405&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 117881405 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 15361632 KJU jtl: Molecular Imaging & Biology issn: 15361632 maglogo: N pubinfo: dt: Oct2016 vid: 18 iid: 5 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 117881405 117881405 NLM26943129 117881405 10.1007/s11307-016-0932-2 NLM26943129 PMC5010961 [Available on 10/01/16] 117881405 ppf: 677 ppct: 9 formats: fmt: @attributes: type: P tig: atl: Detection of Lymph Node Metastases with SERRS Nanoparticles. aug: au: Spaliviero, Massimiliano Harmsen, Stefan Huang, Ruimin Wall, Matthew Andreou, Chrysafis Eastham, James Touijer, Karim Scardino, Peter Kircher, Moritz Wall, Matthew A Eastham, James A Touijer, Karim A Scardino, Peter T Kircher, Moritz F affil: Department of Radiology , Memorial Sloan Kettering Cancer Center , New York 10065 USA sug: subj: Neoplasm Metastasis Pathology Nanoparticles Spectrum Analysis, Raman Methods Neoplasm Metastasis Diagnosis Light Signal Processing, Computer Assisted Animal Studies Models, Biological Prostatic Neoplasms Pathology Cell Line, Tumor Male Funding Source Mice Male ab: Purpose: The accurate detection of lymph node metastases in prostate cancer patients is important to direct treatment decisions. Our goal was to develop an intraoperative imaging approach to distinguish normal from metastasized lymph nodes. We aimed at developing and testing gold-silica surface-enhanced resonance Raman spectroscopy (SERRS) nanoparticles that demonstrate high uptake within normal lymphatic tissue and negligible uptake in areas of metastatic replacement.Procedures: We evaluated the ability of SERRS nanoparticles to delineate lymph node metastases in an orthotopic prostate cancer mouse model using PC-3 cells transduced with mCherry fluorescent protein. Tumor-bearing mice (n = 6) and non-tumor-bearing control animals (n = 4) were injected intravenously with 30 fmol/g SERRS nanoparticles. After 16-18 h, the retroperitoneal lymph nodes were scanned in situ and ex vivo with a Raman imaging system and a handheld Raman scanner and data corroborated with fluorescence imaging for mCherry protein expression and histology.Results: The SERRS nanoparticles demonstrated avid homing to normal lymph nodes, but not to metastasized lymph nodes. In cases where lymph nodes were partially infiltrated by tumor cells, the SERRS signal correctly identified, with sub-millimeter precision, healthy from metastasized components.Conclusions: This study serves as a first proof-of-principle that SERRS nanoparticles enable high precision and rapid intraoperative discrimination between normal and metastasized lymph nodes. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|