Riboflavin and ultraviolet illumination affects selected platelet mRNA transcript amounts differently.
Background: Pathogen inactivation (PI) techniques use ultraviolet (UV) illumination with or without a photosensitizer to destroy pathogen RNA and DNA. Although lacking a nucleus and innate DNA transcription, platelets (PLTs) contain RNA and can synthesize proteins. The impact of PI on PLT protein sy...
| Published in: | Transfusion Vol. 56; no. 9; pp. 2286 - 2296 |
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| Main Authors: | , , |
| Format: | research tables/charts Journal Article |
| Published: |
Wiley-Blackwell
Sep2016
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=118054336&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 118054336 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00411132 1YB jtl: Transfusion issn: 00411132 maglogo: Y pubinfo: dt: Sep2016 vid: 56 iid: 9 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 118054336 118054336 NLM27443848 118054336 10.1111/trf.13715 NLM27443848 118054336 ppf: 2286 ppct: 10 formats: tig: atl: Riboflavin and ultraviolet illumination affects selected platelet mRNA transcript amounts differently. aug: au: Klein‐Bosgoed, Christa Schubert, Peter Devine, Dana V. affil: Department of Pathology and Laboratory Medicine and Centre for Blood Research, University of British Columbia sug: subj: Ultraviolet Rays Riboflavin Pharmacodynamics RNA Blood Platelets Metabolism Glycoproteins Glycoproteins Analysis Antigens, Surface Blood Preservation Methods RNA Radiation Effects Oxidoreductases Analysis RNA Drug Effects Oxidoreductases Antigens, Surface Analysis Blood Platelets Radiation Effects Blood Platelets Drug Effects Human ab: Background: Pathogen inactivation (PI) techniques use ultraviolet (UV) illumination with or without a photosensitizer to destroy pathogen RNA and DNA. Although lacking a nucleus and innate DNA transcription, platelets (PLTs) contain RNA and can synthesize proteins. The impact of PI on PLT protein synthesis and function is unknown; altered synthesis may affect overall PLT quality. In this study we determine to what extent PLT RNA is affected by PI.Study Design and Methods: In a pool-and-split design, paired apheresis PLT concentrates were treated with riboflavin and UV illumination or were left untreated. PLT total RNA and mRNA amounts specific for glycoproteins (GP)IIIa, GPIIb, and GPIb; α-granule proteins PLT factor (PF)4; osteonectin and thrombospondin (TSP); and housekeeping protein glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were determined using absorbance and quantitative polymerase chain reaction.Results: After treatment, amounts of all analyzed mRNAs were significantly reduced (p < 0.05), but to different degrees. For GAPDH and PF4, transcripts appeared less susceptible to the treatment, with 70% remaining 1 hour after UV illumination. For GPIIIa and TSP, less than 15% remained after treatment. There was a correlation (R(2) = 0.85) between transcript length and amount of mRNA remaining 1 hour after treatment. Total RNA demonstrated a life span equal to the PLT life span of 10 to 11 days.Conclusion: This is the first report of the impact of riboflavin and UV illumination on PLT mRNA. Results suggest that all mRNA present in PLTs is affected by the treatment although the degree of the effect varies among transcripts. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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