Whole-transcriptome analysis of chordoma of the skull base.
Fourteen skull base chordoma specimens and three normal specimens were microdissected from paraffin-embedded tissue. Pools of RNA from highly enriched preparations of these cell types were subjected to expression profiling using whole-transcriptome shotgun sequencing. Using strict criteria, 294 diff...
| Publicado en: | Virchows Archiv: European Journal of Pathology Vol. 469; no. 4; pp. 439 - 450 |
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| Autores principales: | , , , , , , , |
| Formato: | Journal Article |
| Publicado: |
Springer Nature
Oct2016
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=118280624&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 118280624 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 09456317 O1Z jtl: Virchows Archiv: European Journal of Pathology issn: 09456317 maglogo: N pubinfo: dt: Oct2016 vid: 469 iid: 4 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 118280624 118280624 NLM27401718 10.1007/s00428-016-1985-y NLM27401718 118280624 ppf: 439 ppct: 11 formats: fmt: @attributes: type: P tig: atl: Whole-transcriptome analysis of chordoma of the skull base. aug: au: Bell, Diana Raza, Shaan Bell, Achim Fuller, Gregory DeMonte, Franco Raza, Shaan M Bell, Achim H Fuller, Gregory N affil: Lone Star College , Houston USA sug: subj: Neoplasms, Germ Cell and Embryonal Diagnosis Skull Neoplasms Metabolism Neoplasms, Germ Cell and Embryonal Metabolism Genes Gene Expression Profiling Methods Skull Neoplasms Pathology Middle Age Aged Adolescence Male Neoplasms, Germ Cell and Embryonal Prognosis Child Female Adult Middle Aged: 45-64 years Aged: 65+ years Adolescent: 13-18 years Child: 6-12 years Adult: 19-44 years Male Female ab: Fourteen skull base chordoma specimens and three normal specimens were microdissected from paraffin-embedded tissue. Pools of RNA from highly enriched preparations of these cell types were subjected to expression profiling using whole-transcriptome shotgun sequencing. Using strict criteria, 294 differentially expressed transcripts were found, with 28 % upregulated and 72 % downregulated. The transcripts were annotated using NCBI Entrez Gene and computationally analyzed with the Ingenuity Pathway Analysis program. From these significantly changed expressions, the analysis identified 222 cancer-related transcripts. These 294 differentially expressed genes and non-coding RNA transcripts provide here a set to specifically define skull base chordomas and to identify novel and potentially important targets for diagnosis, prognosis, and therapy of this cancer. Significance Genomic profiling to subtype skull base chordoma reveals potential candidates for specific biomarkers, with validation by IHC for selected candidates. The highly expressed developmental genes T, LMX1A, ZIC4, LHX4, and HOXA1 may be potential drivers of this disease. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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