Anti-ulcer mechanisms of polyphenols extract of Euphorbia umbellata (Pax) Bruyns (Euphorbiaceae).
Ethnopharmacological relevance Euphorbia umbellata ( leitosinha ) is used in southern Brazilian folk medicine to treat gastric problems, as well as for its analgesic and anti-inflammatory properties. Aim of study To evaluate the anti-ulcer effects of methanolic bark fraction (MF) against in vivo and...
| Published in: | Journal of Ethnopharmacology Vol. 191; pp. 29 - 41 |
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| Main Authors: | , , , , , , , , , , , , , |
| Format: | research Journal Article |
| Published: |
Elsevier B.V.
Sep2016
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=118341472&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 118341472 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 03788741 3MS jtl: Journal of Ethnopharmacology issn: 03788741 maglogo: N pubinfo: dt: Sep2016 vid: 191 pid: 1004 pub: Elsevier B.V. artinfo: ui: 118341472 118341472 118341472 10.1016/j.jep.2016.06.032 118341472 ppf: 29 ppct: 12 formats: tig: atl: Anti-ulcer mechanisms of polyphenols extract of Euphorbia umbellata (Pax) Bruyns (Euphorbiaceae). aug: au: Minozzo, Bruno Rodrigo Lemes, Bruna Mikulis Justo, Aline da Silva Lara, Jheniffer Ellen Petry, Victor Emanuel Kubaski Fernandes, Daniel Belló, Caroline Vellosa, José Carlos Rebuglio Campagnoli, Eduardo Bauml Nunes, Otalíbio Castiglione Kitagawa, Rodrigo Rezende Avula, Bharathi Khan, Ikhlas Ahmad Beltrame, Flávio Luis affil: Department of Pharmaceutical Sciences, State University of Ponta Grossa, Ponta Grossa 84900–030, Paraná, Brazil sug: subj: Plants, Medicinal Brazil Plant Extracts Pharmacodynamics Antiulcer Agents Pharmacodynamics Antioxidants Pharmacodynamics Antibiotics Pharmacodynamics Plant Extracts Analysis Brazil Alternative Therapies In Vivo Studies Animal Studies Peptic Ulcer Chemically Induced Histological Techniques Plant Bark Plant Extracts Administration and Dosage In Vitro Studies Microbial Culture and Sensitivity Tests Chromatography, Liquid Helicobacter Pylori Urease Antagonists and Inhibitors Mass Spectrometry Models, Biological Descriptive Statistics Prostaglandins Metabolism Nitric Oxide Metabolism Glutathione Metabolism Rodents Phytochemicals Analysis Flavonols Analysis Plant Stems Phenols Analysis ab: Ethnopharmacological relevance Euphorbia umbellata ( leitosinha ) is used in southern Brazilian folk medicine to treat gastric problems, as well as for its analgesic and anti-inflammatory properties. Aim of study To evaluate the anti-ulcer effects of methanolic bark fraction (MF) against in vivo and in vitro assays, as well as an antioxidant, antibacterial and chromatographic study of this fraction. Materials and methods In vivo anti-ulcer activity was performed using ethanol and indomethacin models with different MF concentrations (50, 100 or 200 mg/Kg). The stomachs of the animals were applied to histological evaluation, and the serum to evaluate the ABTS •+ radical capture. The 200 mg/Kg dose was used to analyze the mechanisms involved in antiulcerogenic properties of methanolic fraction. The in vitro activity was performed using several different antioxidant assays, in addition to anti- Helicobacter pylori and anti-urease experiments. The chromatographic study was carried out by LC-MS analysis. Results Pharmacological investigation of the MF showed an anti-ulcer potential in ethanol and indomethacin in vivo assays. The material presented a high antioxidant activity for several oxidant in vitro systems (DPPH • , ABTS •+ , O 2 •- , HOCl, TauCl and HRP), as well as an ABTS •+ capture increasing (7.5%) by the treated animals serum (when compared to the negative control). Prostaglandins, nitric oxide/ cyclic guanosine monophosphate pathway and involvement of the protein components of the glutathione complex are some of the mechanisms related with this potential anti-ulcer action. The histological examination of the stomachs of the animals showed that the MF also prevents local action of offensive agents. Chemical analysis using LC-QTOF-MS revealed the presence of ellagic and gallic acid derivatives and flavonols. Conclusion The findings provide scientific basis to the ethnopharmacological purpose of the studied plant and the biological activities of MF of E. umbellata stem bark may be due to the presence of phenolic compounds. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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