Loop-Mediated Isothermal Amplification as a Fast Noninvasive Method of Helicobacter pylori Diagnosis.
Background: Helicobacter pylori infection is etiologically associated with some important health problems such as gastric cancer. Because of the high clinical importance of H. pylori infection, development of a noninvasive test for the detection of H. pylori is desirable.Methods: In this study, a lo...
| Publicado en: | Journal of Clinical Laboratory Analysis Vol. 30; no. 5; pp. 464 - 471 |
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| Autores principales: | , , , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
Sep2016
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=118441121&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 118441121 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 08878013 OCK jtl: Journal of Clinical Laboratory Analysis issn: 08878013 maglogo: Y pubinfo: dt: Sep2016 vid: 30 iid: 5 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 118441121 118441121 NLM26351213 118441121 10.1002/jcla.21880 NLM26351213 118441121 ppf: 464 ppct: 7 formats: tig: atl: Loop-Mediated Isothermal Amplification as a Fast Noninvasive Method of Helicobacter pylori Diagnosis. aug: au: Yari, Farideh Abiri, Ramin Aryan, Ehsan Ahmadi Jouybari, Touraj Navabi, Jafar Alvandi, Amirhooshang affil: Department of Microbiology, Islamic Azad University, Qom Branch, Qom Iran sug: subj: Helicobacter Pylori Nucleic Acid Amplification Techniques Methods Helicobacter Infections Diagnosis Microbiological Techniques Adolescence Female Helicobacter Infections Metabolism Aged Adult Young Adult Documentation Middle Age Antigens, Bacterial Male Aged, 80 and Over Feces Sensitivity and Specificity Feces Microbiology Antigens, Bacterial Analysis Human Adolescent: 13-18 years Aged: 65+ years Adult: 19-44 years Middle Aged: 45-64 years Aged, 80 & over Female Male ab: Background: Helicobacter pylori infection is etiologically associated with some important health problems such as gastric cancer. Because of the high clinical importance of H. pylori infection, development of a noninvasive test for the detection of H. pylori is desirable.Methods: In this study, a loop-mediated isothermal amplification (LAMP) targeted ureC of H. pylori was evaluated on 100 stool specimens and compared with a stool antigen test. Culture and rapid urease test were considered as gold standards.Results: The overall detection rate of the fecal antigen test and LAMP was 58% and 82%, respectively. The analytical sensitivity of the fecal antigen test and LAMP was 500 and 10 H. pylori cells/g and 10 fg DNA/reaction, which is equal to six H. pylori genome.Conclusion: LAMP technique has been characterized by high sensitivity and low detection limit for the detection of H. pylori in stool specimen. Clinical diagnostic performance of LAMP was better than the stool antigen test. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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