Discovery of Potential Inhibitors of Aldosterone Synthase from Chinese Herbs Using Pharmacophore Modeling, Molecular Docking, and Molecular Dynamics Simulation Studies.

Aldosterone synthase (CYP11B2) is a key enzyme for the biosynthesis of aldosterone, which plays a significant role for the regulation of blood pressure. Excess aldosterone can cause the dysregulation of the renin-angiotensin-aldosterone system (RAAS) and lead to hypertension. Therefore, research and...

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Publicado en:BioMed Research International Vol. 2016; pp. 1 - 9
Autores principales: Luo, Ganggang, Lu, Fang, Qiao, Liansheng, Chen, Xi, Li, Gongyu, Zhang, Yanling
Formato: pictorial research tables/charts Journal Article
Publicado: Wiley-Blackwell 10/3/2016
Acceso en línea:Ver este registro en EBSCOhost
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      dt: 10/3/2016
      vid: 2016
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      pub: Wiley-Blackwell
      place: Malden, Massachusetts
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        10.1155/2016/4182595
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        atl: Discovery of Potential Inhibitors of Aldosterone Synthase from Chinese Herbs Using Pharmacophore Modeling, Molecular Docking, and Molecular Dynamics Simulation Studies.
      aug:
        au:
          Luo, Ganggang
          Lu, Fang
          Qiao, Liansheng
          Chen, Xi
          Li, Gongyu
          Zhang, Yanling
        affil: Beijing Key Laboratory of TCM Foundation and New Drug Research, School of Chinese Material Medica, Beijing University of Chinese Medicine, Beijing 100102, China
      sug:
        subj:
          Oxidoreductases Antagonists and Inhibitors
          Drugs, Chinese Herbal Analysis
          Models, Theoretical
          Simulations
          Aldosterone
          Pharmacophore
          Hypertension Drug Therapy
          Validity
          Molecular Structure
          Resource Databases
          Imidazoles Analysis
          Acids Analysis
          Descriptive Statistics
          Funding Source
      ab: Aldosterone synthase (CYP11B2) is a key enzyme for the biosynthesis of aldosterone, which plays a significant role for the regulation of blood pressure. Excess aldosterone can cause the dysregulation of the renin-angiotensin-aldosterone system (RAAS) and lead to hypertension. Therefore, research and development of CYP11B2 inhibitor are regarded as a novel approach for the treatment of hypertension. In this study, the pharmacophore models of CYP11B2 inhibitors were generated and the optimal model was used to identify potential CYP11B2 inhibitors from the Traditional Chinese Medicine Database (TCMD, Version 2009). The hits were further refined by molecular docking and the interactions between compounds and CYP11B2 were analyzed. Compounds with high Fitvalue, high docking score, and expected interactions with key residues were selected as potential CYP11B2 inhibitors. Two most promising compounds, ethyl caffeate and labiatenic acid, with high Fitvalue and docking score were reserved for molecular dynamics (MD) study. All of them have stability of ligand binding which suggested that they might perform the inhibitory effect on CYP11B2. This study provided candidates for novel drug-like CYP11B2 inhibitors by molecular simulation methods for the hypertension treatment.
      pubtype: Academic Journal
      doctype:
        pictorial
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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