A study of hearing function and histopathologic changes in the cochlea of the type 2 diabetes model Tsumura Suzuki obese diabetes mouse.
Objectives:This study used Tsumura Suzuki Obese Diabetes (TSOD) mice as a spontaneous type 2 diabetes model and Tsumura Suzuki Non-obesity (TSNO) mice as controls to investigate factors involved in the onset of hearing impairment. Method:Body weight, blood glucose levels, and auditory brainstem resp...
| Publicado en: | Acta Oto-Laryngologica Vol. 136; no. 11; pp. 1097 - 1107 |
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| Autores principales: | , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Taylor & Francis Ltd
Nov2016
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=118669907&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 118669907 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00016489 B6D jtl: Acta Oto-Laryngologica issn: 00016489 maglogo: Y pubinfo: dt: Nov2016 vid: 136 iid: 11 pid: 377 pub: Taylor & Francis Ltd place: Philadelphia, Pennsylvania artinfo: ui: 118669907 118669907 118669907 10.1080/00016489.2016.1195012 118669907 ppf: 1097 ppct: 10 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: A study of hearing function and histopathologic changes in the cochlea of the type 2 diabetes model Tsumura Suzuki obese diabetes mouse. aug: au: Tsuda, Junko Sugahara, Kazuma Hori, Takeshi Kanagawa, Eiju Takaki, Eiichi Fujimoto, Mitsuaki Nakai, Akira Yamashita, Hiroshi affil: Department of Otolaryngology, Yamaguchi University Graduate School of Medicine, Ube City, Yamaguchi Prefecture, Japan sug: subj: Hearing Disorders Cochlea Diabetes Mellitus, Type 2 Obesity Animal Studies Mice Histological Techniques In Vivo Studies In Vitro Studies Staining and Labeling Microscopy Descriptive Statistics Data Analysis Software Microarray Analysis Mann-Whitney U Test Pearson's Correlation Coefficient Fisher's Exact Test Funding Source ab: Objectives:This study used Tsumura Suzuki Obese Diabetes (TSOD) mice as a spontaneous type 2 diabetes model and Tsumura Suzuki Non-obesity (TSNO) mice as controls to investigate factors involved in the onset of hearing impairment. Method:Body weight, blood glucose levels, and auditory brainstem responses (ABRs) were measured. The cochleae were excised and evaluated histopathologically. Results:The TSOD mice showed significant hyperglycemia at 2–7 months and severe obesity at 5–10 months; significantly elevated ABR thresholds at 8–10 months; and the capillary lumens in the cochlea stria vascularis were narrower in the TSOD mice than in the TSNO mice. At 17 months, India ink vascular staining of the TSOD mice’s cochleae revealed decreased capillary density in the stria vascularis. The vascular area of capillaries in the stria vascularis and the vascular area were significantly smaller in TSOD mice. Histopathological analysis showed vessel wall thickening in the modiolus and narrowed capillaries in the stria vascularis, suggesting reduced blood flow to the inner ear. Conclusion:The diabetes mice model used in our study showed early age-associated hearing loss, and histopathology showed findings of vessel wall thickening in the modiolus, narrowing of capillaries in the stria vascularis, and chronically reduced blood flow in the cochlea. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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