Molecular Role of EGFR-MAPK Pathway in Patchouli Alcohol-Induced Apoptosis and Cell Cycle Arrest on A549 Cells In Vitro and In Vivo.
Nowadays, chemotherapy is still the main effective treatment for cancer. Herb prescriptions containing Pogostemon cablin Benth (also known as “Guang-Huo-Xiang”) have been widely used in Chinese medicine today. In our research, we found that patchouli alcohol, a compound isolated from the oil of Pogo...
| Publicado en: | BioMed Research International Vol. 2016; pp. 1 - 13 |
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| Autores principales: | , , , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
10/17/2016
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=118852745&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 118852745 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 10/17/2016 vid: 2016 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 118852745 118852745 118852745 10.1155/2016/4567580 118852745 ppf: 1 ppct: 12 formats: fmt: @attributes: type: P tig: atl: Molecular Role of EGFR-MAPK Pathway in Patchouli Alcohol-Induced Apoptosis and Cell Cycle Arrest on A549 Cells In Vitro and In Vivo. aug: au: Lu, XinGang Yang, Liu Lu, ChengHua Xu, ZhenYu Qiu, HongFu Wu, JiaJia Wang, JingWen Tong, JiaFeng Zhu, Yin Shen, Jie affil: Department of Traditional Chinese Medicine, Huadong Hospital, Fudan University, Shanghai 200040, China sug: subj: Apoptosis Cell Cycle Receptors, Cell Surface Epidermal Growth Factors Protein Kinases Signal Transduction Plants, Medicinal Ethanol Administration and Dosage Terpenes Administration and Dosage Antineoplastic Agents Analysis Human Cell Culture Techniques In Vitro Studies In Vivo Studies Biological Assay Staining and Labeling Molecular Structure Peptide Hydrolases Cell Physiology Mitochondria Lung Neoplasms Drug Therapy Descriptive Statistics Flow Cytometry Immunohistochemistry One-Way Analysis of Variance Post Hoc Analysis P-Value Data Analysis Software Animal Studies Mice Funding Source ab: Nowadays, chemotherapy is still the main effective treatment for cancer. Herb prescriptions containing Pogostemon cablin Benth (also known as “Guang-Huo-Xiang”) have been widely used in Chinese medicine today. In our research, we found that patchouli alcohol, a compound isolated from the oil of Pogostemon cablin Benth, exerted antitumor ability against human lung cancer A549 cells ability both in vitro and in vivo. MTT assay was used to assess cell viability. Hoechst 33342 staining and TUNEL cover glass staining provided the visual evidence of apoptosis. Caspase activity measurement showed that patchouli alcohol activated caspase 9 and caspase 3 of mitochondria-mediated apoptosis. Consistently, patchouli alcohol inhibited the xenograft tumor in vivo. Further investigation of the underlying molecular mechanism showed that MAPK and EGFR pathway might contribute to the antitumor effect of patchouli alcohol. Our study proved that patchouli alcohol might be able to serve as a novel antitumor compound in the clinical treatment of lung cancer. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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