Intraoperative Raman spectroscopy of soft tissue sarcomas.

Background and Objective: Soft tissue sarcomas (STS) are a rare and heterogeneous group of malignant tumors that are often treated through surgical resection. Current intraoperative margin assessment methods are limited and highlight the need for an improved approach with respect to time and specifi...

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Publicado en:Lasers in Surgery & Medicine Vol. 48; no. 8; pp. 774 - 782
Autores principales: Nguyen, John Q., Gowani, Zain S., O'Connor, Maggie, Pence, Isaac J., Nguyen, The‐Quyen, Holt, Ginger E., Schwartz, Herbert S., Halpern, Jennifer L., Mahadevan‐Jansen, Anita
Formato: research Journal Article
Publicado: Wiley-Blackwell Oct2016
Acceso en línea:Ver este registro en EBSCOhost
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      jtl: Lasers in Surgery & Medicine
      issn: 01968092
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    pubinfo:
      dt: Oct2016
      vid: 48
      iid: 8
      pid: 480
      pub: Wiley-Blackwell
      place: Malden, Massachusetts
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        10.1002/lsm.22564
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        atl: Intraoperative Raman spectroscopy of soft tissue sarcomas.
      aug:
        au:
          Nguyen, John Q.
          Gowani, Zain S.
          O'Connor, Maggie
          Pence, Isaac J.
          Nguyen, The‐Quyen
          Holt, Ginger E.
          Schwartz, Herbert S.
          Halpern, Jennifer L.
          Mahadevan‐Jansen, Anita
        affil: Biophotonics Center, Vanderbilt University, 410 24th Ave. South (Keck FEL Center), Nashville Tennessee 37232
      sug:
        subj:
          Intraoperative Care Methods
          Spectrum Analysis, Raman
          Sarcoma Diagnosis
          Soft Tissue Neoplasms Diagnosis
          Adult
          Human
          Sensitivity and Specificity
          Algorithms
          Logistic Regression
          Sarcoma Surgery
          Multivariate Analysis
          Soft Tissue Neoplasms Surgery
          Validation Studies
          Comparative Studies
          Evaluation Research
          Multicenter Studies
          Funding Source
          Adult: 19-44 years
      ab: Background and Objective: Soft tissue sarcomas (STS) are a rare and heterogeneous group of malignant tumors that are often treated through surgical resection. Current intraoperative margin assessment methods are limited and highlight the need for an improved approach with respect to time and specificity. Here we investigate the potential of near-infrared Raman spectroscopy for the intraoperative differentiation of STS from surrounding normal tissue.Materials and Methods: In vivo Raman measurements at 785 nm excitation were intraoperatively acquired from subjects undergoing STS resection using a probe based spectroscopy system. A multivariate classification algorithm was developed in order to automatically identify spectral features that can be used to differentiate STS from the surrounding normal muscle and fat. The classification algorithm was subsequently tested using leave-one-subject-out cross-validation.Results: With the exclusion of well-differentiated liposarcomas, the algorithm was able to classify STS from the surrounding normal muscle and fat with a sensitivity and specificity of 89.5% and 96.4%, respectively.Conclusion: These results suggest that single point near-infrared Raman spectroscopy could be utilized as a rapid and non-destructive surgical guidance tool for identifying abnormal tissue margins in need of further excision. Lasers Surg. Med. 48:774-781, 2016. © 2016 Wiley Periodicals, Inc.
      pubtype: Academic Journal
      doctype:
        research
        Journal Article
      ougenre: Article
    language: English
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