Evaluation of standardized sample collection, packaging, and decontamination procedures to assess cross-contamination potential during Bacillus anthracis incident response operations.
Sample collection procedures and primary receptacle (sample container and bag) decontamination methods should prevent contaminant transfer between contaminated and non-contaminated surfaces and areas during bio-incident operations. Cross-contamination of personnel, equipment, or sample containers ma...
| Publicado en: | Journal of Occupational & Environmental Hygiene Vol. 13; no. 12; pp. 980 - 993 |
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| Autores principales: | , , , , , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Taylor & Francis Ltd
Dec2016
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=119092977&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 119092977 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 15459624 V1L jtl: Journal of Occupational & Environmental Hygiene issn: 15459624 maglogo: Y pubinfo: dt: Dec2016 vid: 13 iid: 12 pid: 377 pub: Taylor & Francis Ltd place: Philadelphia, Pennsylvania artinfo: ui: 119092977 119092977 119092977 10.1080/15459624.2016.1200725 119092977 ppf: 980 ppct: 13 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Evaluation of standardized sample collection, packaging, and decontamination procedures to assess cross-contamination potential during Bacillus anthracis incident response operations. aug: au: Calfee, M. Worth Tufts, Jenia Meyer, Kathryn McConkey, Katrina Mickelsen, Leroy Rose, Laura Dowell, Chad Delaney, Lisa Weber, Angela Morse, Stephen Chaitram, Jasmine Gray, Marshall affil: US Environmental Protection Agency, National Homeland Security Research Center, Research Triangle Park, North Carolina sug: subj: Bacillus Anthrax Bacillaceae Infections Specimen Handling Methods Human Specimen Handling Evaluation Bacterial Contamination ab: Sample collection procedures and primary receptacle (sample container and bag) decontamination methods should prevent contaminant transfer between contaminated and non-contaminated surfaces and areas during bio-incident operations. Cross-contamination of personnel, equipment, or sample containers may result in the exfiltration of biological agent from the exclusion (hot) zone and have unintended negative consequences on response resources, activities and outcomes. The current study was designed to: (1) evaluate currently recommended sample collection and packaging procedures to identify procedural steps that may increase the likelihood of spore exfiltration or contaminant transfer; (2) evaluate the efficacy of currently recommended primary receptacle decontamination procedures; and (3) evaluate the efficacy of outer packaging decontamination methods. Wet- and dry-deposited fluorescent tracer powder was used in contaminant transfer tests to qualitatively evaluate the currently-recommended sample collection procedures.Bacillus atrophaeusspores, a surrogate forBacillus anthracis, were used to evaluate the efficacy of spray- and wipe-based decontamination procedures. Both decontamination procedures were quantitatively evaluated on three types of sample packaging materials (corrugated fiberboard, polystyrene foam, and polyethylene plastic), and two contamination mechanisms (wet or dry inoculums). Contaminant transfer results suggested that size-appropriate gloves should be worn by personnel, templates should not be taped to or removed from surfaces, and primary receptacles should be selected carefully. The decontamination tests indicated that wipe-based decontamination procedures may be more effective than spray-based procedures; efficacy was not influenced by material type but was affected by the inoculation method. Incomplete surface decontamination was observed in all tests with dry inoculums. This study provides a foundation for optimizing currentB. anthracisresponse procedures to minimize contaminant exfiltration. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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