Optimizing Workflows and Processing of Cytologic Samples for Comprehensive Analysis by Next-Generation Sequencing.
* The value and suitability of cytology specimens for molecular diagnosis has been demonstrated by numerous studies. In practice, however, the success rates vary widely across institutions depending on the disease setting, institutional practices of acquisition, handling/processing, and testing meth...
| Publicado en: | Archives of Pathology & Laboratory Medicine Vol. 140; no. 11; pp. 1200 - 1206 |
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| Autores principales: | , , , , , , , |
| Formato: | pictorial tables/charts Journal Article |
| Publicado: |
College of American Pathologists
Nov2016
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=119227545&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 119227545 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00039985 1FS jtl: Archives of Pathology & Laboratory Medicine issn: 00039985 maglogo: N pubinfo: dt: Nov2016 vid: 140 iid: 11 pid: 2550 pub: College of American Pathologists place: Northfield, Illinois artinfo: ui: 119227545 119227545 119227545 10.5858/arpa.2016-0108-RA 119227545 ppf: 1200 ppct: 6 formats: fmt: @attributes: type: P tig: atl: Optimizing Workflows and Processing of Cytologic Samples for Comprehensive Analysis by Next-Generation Sequencing. aug: au: Shaozhou Ken Tian Killian, J. Keith Rekhtman, Natasha Benayed, Ryma Middha, Sumit Ladanyi, Marc Lin, Oscar Arcila, Maria E. affil: Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, New York sug: subj: Histocytological Preparation Techniques Neoplasms Pathology Sequence Analysis Methods Specimen Handling Methods Cancer Care Facilities New York New York Molecular Diagnostic Techniques Bioinformatics ab: * The value and suitability of cytology specimens for molecular diagnosis has been demonstrated by numerous studies. In practice, however, the success rates vary widely across institutions depending on the disease setting, institutional practices of acquisition, handling/processing, and testing methodologies. As the number of clinically relevant biomarkers continues to increase, more laboratories are turning to next-generation sequencing platforms for testing. Although amplicon-based next-generation sequencing assays, interrogating a limited genomic territory, can be performed with minimal input material, broader-based next-generation sequencing assays have higher DNA input requirements that may not be met if the small tissue samples are not acquired and handled appropriately. We briefly describe some of the process changes we have instituted in our laboratories when handling cytologic material to maximize the tissue available for broad hybrid-capture--based next-generation sequencing assays. Among the key changes established were the consolidation and preservation of previously discarded supernatant material in cytologic samples, the introduction of mineral oil for deparaffinization of cell blocks, and adjustments in the molecular laboratory process and bioinformatics pipelines. We emphasize that even minimal changes can have broad implications for test performance, highlighting the importance of a cohesive group-based approach among clinical, cytopathology, surgical pathology, molecular, and bioinformatics teams. pubtype: Academic Journal doctype: pictorial tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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