Effects of Glycosylation on Biodistribution and Imaging Quality of Necrotic Myocardium of Iodine-131-Labeled Sennidins.
Purpose: Sennidins are necrosis-avid agents for noninvasive assessment of myocardial viability which is important for patients with myocardial infarction (MI). However, high accumulation of radioactivity in the liver interferes with the assessment of myocardial viability. In this study, we compared...
| Published in: | Molecular Imaging & Biology Vol. 18; no. 6; pp. 877 - 887 |
|---|---|
| Main Authors: | , , , , , , , , , , , |
| Format: | pictorial research tables/charts Journal Article |
| Published: |
Springer Nature
Dec2016
|
| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=119233700&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 119233700 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 15361632 KJU jtl: Molecular Imaging & Biology issn: 15361632 maglogo: N pubinfo: dt: Dec2016 vid: 18 iid: 6 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 119233700 119233700 NLM27172937 119233700 10.1007/s11307-016-0961-x NLM27172937 119233700 ppf: 877 ppct: 10 formats: fmt: @attributes: type: P tig: atl: Effects of Glycosylation on Biodistribution and Imaging Quality of Necrotic Myocardium of Iodine-131-Labeled Sennidins. aug: au: Li, Ling Zhang, Dongjian Yang, Shengwei Song, Shaoli Li, Jindian Wang, Qin Wang, Cong Feng, Yuanbo Ni, Yicheng Zhang, Jian Liu, Wei Yin, Zhiqi affil: Department of Nuclear Medicine, Renji Hospital , Shanghai Jiaotong University, School of Medicine , Shanghai 200127 People's Republic of China sug: subj: Plant Extracts Tomography, X-Ray Computed Myocardium Pathology Tomography, Emission-Computed, Single-Photon Iodine Radioisotopes Hydrocarbons Alcohols Radiography Glycosylation Time Factors Cell Physiology Necrosis Rats Animal Studies Staining and Labeling Plant Extracts Pharmacokinetics Hydrocarbons Pharmacokinetics Water Molecular Structure ab: Purpose: Sennidins are necrosis-avid agents for noninvasive assessment of myocardial viability which is important for patients with myocardial infarction (MI). However, high accumulation of radioactivity in the liver interferes with the assessment of myocardial viability. In this study, we compared sennidins with sennosides to investigate the effects of glycosylation on biodistribution and imaging quality of sennidins.Procedures: Sennidin A (SA), sennidin B (SB), sennoside A (SSA), and sennoside B (SSB) were labeled with I-131. In vitro binding to necrotic cells and hepatic cells and in vivo biodistribution in rats with muscular necrosis were evaluated by gamma counting, autoradiography, and histopathology. Single photon emission computed tomography/computed tomography (SPECT/CT) images were acquired in rats with acute MI.Results: The uptake of [131I]SA, [131I]SSA, [131I]SB, and [131I]SSB in necrotic cells was significantly higher than that in viable cells (p < 0.05). Hepatic cells uptake of [131I]SSA and [131I]SSB were 7-fold and 10-fold lower than that of corresponding [131I]SA and [131I]SB, respectively. The biodistribution data showed that the radioactivities in the liver and feces were significantly lower with [131I]sennosides than those with [131I]sennidins (p < 0.01). Autoradiography showed preferential accumulation of these four radiotracers in necrotic areas of muscle, confirmed by histopathology. SPECT/CT imaging studies showed better image quality with [131I]SSB than with [131I]SB due to less liver interference.Conclusions: Glycosylation significantly decreased the liver uptake and improved the quality of cardiac imaging. [131I]SSB may serve as a promising necrosis-avid agent for noninvasive assessment of myocardial viability. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|