A Clinical Wide-Field Fluorescence Endoscopic Device for Molecular Imaging Demonstrating Cathepsin Protease Activity in Colon Cancer.
Purpose: Early and effective detection of cancers of the gastrointestinal tract will require novel molecular probes and advances in instrumentation that can reveal functional changes in dysplastic and malignant tissues. Here, we describe adaptation of a wide-field clinical fiberscope to perform wide...
| Publicado en: | Molecular Imaging & Biology Vol. 18; no. 6; pp. 820 - 830 |
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| Autores principales: | , , , , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Springer Nature
Dec2016
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=119233703&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 119233703 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 15361632 KJU jtl: Molecular Imaging & Biology issn: 15361632 maglogo: N pubinfo: dt: Dec2016 vid: 18 iid: 6 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 119233703 119233703 NLM27154508 119233703 10.1007/s11307-016-0956-7 NLM27154508 119233703 ppf: 820 ppct: 10 formats: fmt: @attributes: type: P tig: atl: A Clinical Wide-Field Fluorescence Endoscopic Device for Molecular Imaging Demonstrating Cathepsin Protease Activity in Colon Cancer. aug: au: Sensarn, Steven Zavaleta, Cristina Segal, Ehud Rogalla, Stephan Lee, Wansik Gambhir, Sanjiv Bogyo, Matthew Contag, Christopher Zavaleta, Cristina L Gambhir, Sanjiv S Contag, Christopher H affil: Department of Pathology , Stanford University , Stanford 94305 USA sug: subj: Endoscopy Equipment and Supplies Molecular Imaging Equipment and Supplies Colonic Neoplasms Peptide Hydrolases Metabolism Microscopy Colonic Neoplasms Pathology Light Colon Pathology Mice Colonic Polyps Pathology Animal Studies Funding Source ab: Purpose: Early and effective detection of cancers of the gastrointestinal tract will require novel molecular probes and advances in instrumentation that can reveal functional changes in dysplastic and malignant tissues. Here, we describe adaptation of a wide-field clinical fiberscope to perform wide-field fluorescence imaging while preserving its white-light capability for the purpose of providing wide-field fluorescence imaging capability to point-of-care microscopes.Procedures: We developed and used a fluorescent fiberscope to detect signals from a quenched probe, BMV109, that becomes fluorescent when cleaved by, and covalently bound to, active cathepsin proteases. Cathepsins are expressed in inflammation- and tumor-associated macrophages as well as directly from tumor cells and are a promising target for cancer imaging. The fiberscope has a 1-mm outer diameter enabling validation via endoscopic exams in mice, and therefore we evaluated topically applied BMV109 for the ability to detect colon polyps in an azoxymethane-induced colon tumor model in mice.Results: This wide-field endoscopic imaging device revealed consistent and clear fluorescence signals from BMV109 that specifically localized to the polypoid regions as opposed to the normal adjacent colon tissue (p < 0.004) in the murine colon carcinoma model.Conclusions: The sensitivity of detection of BMV109 with the fluorescence fiberscope suggested utility of these tools for early detection at hard-to-reach sites. The fiberscope was designed to be used in conjunction with miniature, endoscope-compatible fluorescence microscopes for dual wide-field and microscopic cancer detection. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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