Genotyping and Mutation Pattern in the Overlapping MHR Region of HBV Isolates in Southern Khorasan, Eastern Iran.
Background: Hepatitis B virus, with 8 known distinct genotypes, is one of the most serious health problems which results to liver injuries. The surface gene of Hepatitis B virus completely overlaps with the polymerase gene. Mutations in the RT gene result in changes in the overlapping hepatitis B su...
| Publicado en: | Hepatitis Monthly Vol. 16; no. 10; pp. 1 - 9 |
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| Autores principales: | , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Kowsar Medical Publishing Company
Oct2016
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=119251872&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 119251872 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 1735143X 780O jtl: Hepatitis Monthly issn: 1735143X maglogo: N pubinfo: dt: Oct2016 vid: 16 iid: 10 pid: 78170 pub: Kowsar Medical Publishing Company place: , <Blank> artinfo: ui: 119251872 119251872 119251872 10.5812/hepatmon.37806 119251872 ppf: 1 ppct: 8 formats: tig: atl: Genotyping and Mutation Pattern in the Overlapping MHR Region of HBV Isolates in Southern Khorasan, Eastern Iran. aug: au: Ziaee, Masood Javanmard, Davod Sharifzadeh, Gholamreza Namaei, Mohammad Hasan Azarkar, Ghodsiyeh affil: Hepatitis Research Center, Birjand University of Medical Sciences, Birjand, IR Iran sug: subj: Mycoplasma Hepatitis B Nucleic Acid Amplification Techniques Mutation Polymerase Chain Reaction Genes Hepatitis Viruses Iran Male Female Male Female ab: Background: Hepatitis B virus, with 8 known distinct genotypes, is one of the most serious health problems which results to liver injuries. The surface gene of Hepatitis B virus completely overlaps with the polymerase gene. Mutations in the RT gene result in changes in the overlapping hepatitis B surface antigen. Objectives: The present study aimed to evaluate the genotypes and prevalence of mutations in a segment of S and RT gene in HBV isolates in Southern Khorasan, Iran. Methods: This was a population-based study comprising 5,235 randomized samples for HBV screening. A nested-polymerase chain reaction (PCR) test was followed by direct sequencing, and the sequences blast with present sequences of NCBI database for genotyping. Alignment and phylogenic analysis was performed using MEGA-6 software, and mutation pattern of this segment was finally surveyed in Bioedit software. Results: The mean age was 39.07 † 14.04 years, with 52.2% female and 47.8% male. All isolates belonged to HBV genotype D, subgenotype D1. The most amino acid substitutions of surface protein were Q129H (34.42%) and A168V (8.2%), other escape mutants observed in this study were P127L/T, S117G, T125M, S143L, D144E and E164D. In the RT gene, Q149K was the most frequently identified amino acid substitution (9.83%), followed by L122F (8.19%), N118D/T (6.55%), L157M (4.91%), and H124Y (3.27%). Conclusions: This finding represents an ongoing dominancy of HBV genotype D in Eastern Iran, corresponding to other parts of Iran. There were a lot of variations in the S gene leading to an escape mutation, some of which affected the corresponding area of the RT region. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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