Evaluation of Selenite F broth as an enrichment step for the isolation of Salmonella and Shigella in clinical faecal specimens -A retrospective study.
Objectives: To investigate whether enrichment of clinical faecal specimens in Selenite F broth, for the cultivation of Salmonella and Shigella, should be discontinued in the community laboratory setting. Methods: A retrospective analysis of all faecal specimens submitted to Aotea Pathology Limited f...
| Publicado en: | New Zealand Journal of Medical Laboratory Science Vol. 70; no. 3; pp. 92 - 95 |
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| Autores principales: | , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
New Zealand Institute of Medical Laboratory Science
Nov2016
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=119547600&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 119547600 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 11710195 H2G jtl: New Zealand Journal of Medical Laboratory Science issn: 11710195 maglogo: N pubinfo: dt: Nov2016 vid: 70 iid: 3 pid: 12822 pub: New Zealand Institute of Medical Laboratory Science artinfo: ui: 119547600 119547600 119547600 119547600 ppf: 92 ppct: 3 formats: fmt: @attributes: type: P tig: atl: Evaluation of Selenite F broth as an enrichment step for the isolation of Salmonella and Shigella in clinical faecal specimens -A retrospective study. aug: au: Donaldson, Arleen Nicol, Mackenzie affil: Aotea Pathology, Wellington sug: subj: Feces Microbiology Culture Techniques Salmonella Shigella Gram-Negative Bacteria Bacterial Colonization Retrospective Design Comparative Studies Culture Media ab: Objectives: To investigate whether enrichment of clinical faecal specimens in Selenite F broth, for the cultivation of Salmonella and Shigella, should be discontinued in the community laboratory setting. Methods: A retrospective analysis of all faecal specimens submitted to Aotea Pathology Limited for bacterial culture over a two-year period was analysed using the Labsolutions statistics software. Samples positive for either Salmonella or Shigella were analysed as being isolated from either the direct Hektoen agar plate, isolated on secondary XLD agar plate following enrichment in Selenite F broth, or both. Results: Direct culture on Hektoen agar yielded a recovery rate of 39% of the Salmonella isolated and 67% of the Shigella isolated. Secondary sub culture on XLD agar following enrichment in Selenite F broth yielded a recovery rate of 99% of the Salmonella isolated and 50% of the Shigella isolated. Conclusions: This study shows that enrichment in Selenite F broth should continue to be used and a combination of direct plating and sub-culturing is required in order to maximise the recovery rate of both Salmonella and Shigella as well as to provide early detection. If enrichment was to be discontinued, potentially a significant proportion of Salmonella and Shigella isolates may have been missed (61% and 33% respectively). pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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