EF24 Suppresses Invasion and Migration of Hepatocellular Carcinoma Cells In Vitro via Inhibiting the Phosphorylation of Src.
Diphenyl difluoroketone (EF24), a curcumin analog, is a promising anticancer compound that exerts its effects by inhibiting cell proliferation and inducing apoptosis. However, the efficacy of EF24 against cancer metastasis, particularly in hepatocellular carcinoma (HCC), remains elusive. In this stu...
| Publicado en: | BioMed Research International Vol. 2016; pp. 1 - 11 |
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| Autores principales: | , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
11/23/2016
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=119732062&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 119732062 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 11/23/2016 vid: 2016 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 119732062 119732062 119732062 10.1155/2016/8569684 119732062 ppf: 1 ppct: 10 formats: fmt: @attributes: type: P tig: atl: EF24 Suppresses Invasion and Migration of Hepatocellular Carcinoma Cells In Vitro via Inhibiting the Phosphorylation of Src. aug: au: Zhao, Ran Tin, Lamtin Zhang, Yuhua Wu, Yiqi Jin, Yinji Jin, Xiaoming Zhang, Fengmin Li, Xiaobo affil: Department of Pathology, Harbin Medical University, Harbin, Heilongjiang 150081, China sug: subj: Carcinoma, Hepatocellular Drug Therapy In Vitro Studies Phosphorylation Drug Effects Neoplasm Invasiveness Drug Therapy Cell Movement Drug Effects Ketones Administration and Dosage Curcumin Administration and Dosage Antineoplastic Agents Analysis Antineoplastic Agents Administration and Dosage Neoplasm Metastasis Drug Therapy Protein-Tyrosine Kinases Drug Effects Molecular Structure Cell Culture Techniques Cell Line, Tumor Wound Healing Biological Assay Cellular Structures Lymph Nodes Tissue Culture Techniques Cell Physiology Drug Effects Apoptosis Drug Effects Microscopy, Electron Blotting, Western Immunohistochemistry Data Analysis Software T-Tests Mann-Whitney U Test P-Value Descriptive Statistics Flow Cytometry Human Chinese Persons Protein-Tyrosine Kinases Analysis Funding Source ab: Diphenyl difluoroketone (EF24), a curcumin analog, is a promising anticancer compound that exerts its effects by inhibiting cell proliferation and inducing apoptosis. However, the efficacy of EF24 against cancer metastasis, particularly in hepatocellular carcinoma (HCC), remains elusive. In this study, the effect of EF24 on HCCLM-3 and HepG2 cell migration and invasion was detected by wound healing and transwell assay, respectively. The results revealed that EF24 suppressed the migration and invasion of both HCCLM-3 and HepG2 cells. Furthermore, EF24 treatment decreased the formation of filopodia on the cell surface and inhibited the phosphorylation of Src in both cell lines, which may help contribute towards understanding the mechanism underlying the suppressive effect of EF24 on HCC migration and invasion. Additionally, the expression of total- and phosphorylated-Src in primary HCC tissues and their paired lymph node metastatic tissues was detected, and phosphorylated-Src was found to be associated with HCC lymph node metastasis. The results of this study suggest that Src is a novel and promising therapeutic target in HCC and provide evidence to support the hypothesis that EF24 may be a useful therapeutic agent for the treatment of HCC. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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