Mineral Formation in the Larval Zebrafish Tail Bone Occurs via an Acidic Disordered Calcium Phosphate Phase.
Both in vivo and ex vivo observations support the hypothesis that bone mineral formation proceeds via disordered precursor phases. The characteristics of the precursor phases are not well defined, but octacalcium phosphate-like, amorphous calcium phosphate-like, and H P 0 42~-enriched phases were de...
| Publicado en: | Journal of the American Chemical Society Vol. 138; no. 43; pp. 14481 - 14488 |
|---|---|
| Autores principales: | , , , , , , , |
| Formato: | Artículo |
| Publicado: |
American Chemical Society
11/2/2016
|
| Materias: | |
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=hlh&AN=119789646&site=ehost-live header: @attributes: shortDbName: hlh uiTerm: 119789646 longDbName: Humanities International Complete uiTag: AN controlInfo: bkinfo: jinfo: jid: 00027863 ACS jtl: Journal of the American Chemical Society issn: 00027863 maglogo: N pubinfo: dt: 11/2/2016 vid: 138 iid: 43 pid: 997 pub: American Chemical Society artinfo: ui: 119789646 10.1021/jacs.6b09442 ppf: 14481 ppct: 7 formats: tig: atl: Mineral Formation in the Larval Zebrafish Tail Bone Occurs via an Acidic Disordered Calcium Phosphate Phase. aug: au: Akiva, Anat Kerschnitzki, Michael Pinkas, Iddo Wagermaier, Wolfgang Yaniv, Karina Fratzl, Peter Addadi, Lia Weiner, Steve affil: Department of Structural Biology, Weizmann Institute of Science, Rehovot 76100, Israel Department of Chemical Research Support, Weizmann Institute of Science, Rehovot 76100, Israel Department of Biomaterials, Max Planck Institute of Colloids and Interfaces, 14476 Potsdam, Germany Department of Biological Regulation, Weizmann Institute of Science, Rehovot76l00, Israel su: Coccyx Zebra danio embryos Calcium phosphate Chemical precursors Ossification Crystallization sug: subj: Coccyx Zebra danio embryos Calcium phosphate Chemical precursors Ossification Crystallization ab: Both in vivo and ex vivo observations support the hypothesis that bone mineral formation proceeds via disordered precursor phases. The characteristics of the precursor phases are not well defined, but octacalcium phosphate-like, amorphous calcium phosphate-like, and H P 0 42~-enriched phases were detected. Here we use in vivo Raman spectroscopy and high-resolution wide-angle X-ray diffraction (WAXD) to characterize and map at 2 jrm resolution the mineral phases in the rapidly forming tail fin bones of living zebrafish larvae and zebrafish larvae immediately after sacrifice, respectively. Raman spectroscopy shows the presence of an acidic disordered calcium phosphate phase with additional characteristic features of HP042 at the bone-cell interface. The complexity in the position and shape of the v x P 0 4 peak viewed by in vivo Raman spectroscopy emphasizes the heterogeneity of the mineral during bone formation. WAXD detects an additional isolated peak, appearing alone or together with the characteristic diffraction pattern of carbonated hydroxyapatite. This unidentified phase is located at the interface between the mature bone and the surrounding tissue, similar to the location at which the disordered phase was observed by Raman spectroscopy. The variable peak positions and profiles support the notion that this is an unstable disordered precursor phase, which conceivably crystallized during the X-ray diffraction measurement. Interestingly, this precursor phase is co-aligned with the c-axes of the mature bone crystals and thus is in intimate relation with the surrounding collagen matrix. We conclude that a major disordered precursor mineral phase containing HP042_ is part of the deposition pathway of the rapidly forming tail fin bones of the zebrafish. pubtype: Academic Journal doctype: Article src: R language: English refInfo: copyright: @attributes: flag: Y dt: @attributes: year: 2016 holdings: @attributes: islocal: N |
|---|