Oxidative Stress Biomarkers in Exhaled Breath of Workers Exposed to Crystalline Silica Dust by SPME-GC-MS.

Background: Silicosis is considered an oxidative stress related disease that can lead to the development of lung cancer. In this study, our purpose was to analysis of volatile organic compounds (VOCs) in the exhaled breath of workers exposed to silica containing dust and compare peak area of these c...

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Publicado en:Journal of Research in Health Sciences Vol. 16; no. 3; pp. 153 - 162
Autores principales: Jalali, Mahdi, Zare Sakhvidi, Mohammad Javad, Bahrami, Abdulrahman, Berijani, Nima, Mahjub, Hussein
Formato: research tables/charts Journal Article
Publicado: Hamadan University of Medical Sciences, School of Public Health Summer2016
Acceso en línea:Ver este registro en EBSCOhost
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      pub: Hamadan University of Medical Sciences, School of Public Health
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        atl: Oxidative Stress Biomarkers in Exhaled Breath of Workers Exposed to Crystalline Silica Dust by SPME-GC-MS.
      aug:
        au:
          Jalali, Mahdi
          Zare Sakhvidi, Mohammad Javad
          Bahrami, Abdulrahman
          Berijani, Nima
          Mahjub, Hussein
        affil: Department of Occupational Health, Social Determinants of Health Research Center, Birjand University of Medical Sciences, Birjand, Iran
      sug:
        subj:
          Occupational Exposure
          Silicon Dioxide
          Breath Tests
          Organic Chemicals Analysis
          Human
          Cross Sectional Studies
          Case Control Studies
          Pneumoconiosis
          Solid Phase Extraction
          Gas Chromatography-Mass Spectrometry
          Inhalation Exposure
          Spirometry
          Oxidative Stress
          Biological Markers
      ab: Background: Silicosis is considered an oxidative stress related disease that can lead to the development of lung cancer. In this study, our purpose was to analysis of volatile organic compounds (VOCs) in the exhaled breath of workers exposed to silica containing dust and compare peak area of these compounds with silicosis patients and healthy volunteers (smokers and nonsmokers) groups. Methods: In this cross sectional case-control study, the exhaled breath of 69 subjects including workers exposed to silica (n=20), silicosis patient (n=4), healthy non-smoker (n=20) and healthy smoker (n=25) were analyzed. We collected breath samples using 3-liter Tedlar bags. The VOCs were extracted with solid phase micro-extraction (SPME) and analyzed by gas chromatography-mass spectrometry (GC-MS). Personal exposure intensity was measured according to NIOSH 7601 method. Respiratory parameters were measured using spirometry. Results: Seventy percent and 100% of the exposures to crystalline silica dust exceeded from 8 h TWA ACGIH TLVs in case and positive control groups, respectively. A significant negative correlation was found between dust exposure intensity and FEV1/FVC when exposure and positive control groups were studied in a group (r2 =-0.601, P<0.001). Totally, forty VOCs were found in all exhaled breath samples. Among the VOCs, the mean of peak area acetaldehyde, hexanal, nonanal, decane, pentad cane, 2-propanol and 3-hydroxy-2-butanone were higher in exhaled breath of the workers exposed to silica and silicosis patient compared to the healthy smoker and nonsmoker controls. In some cases the difference was significant (P<0.05). Conclusions: The analysis of some VOCs in exhaled breath of subjects is appropriate biomarker to determine of exposure to silica.
      pubtype: Academic Journal
      doctype:
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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