An ex vivo porcine skin model to evaluate pressure-reducing devices of different mechanical properties used for pressure ulcer prevention.
Pressure ulcers are complex wounds caused by pressure- and shear-induced trauma to skin and underlying tissues. Pressure-reducing devices, such as dressings, have been shown to successfully reduce pressure ulcer incidence, when used in adjunct to pressure ulcer preventative care. While pressure-redu...
| Published in: | Wound Repair & Regeneration Vol. 24; no. 6; pp. 1089 - 1097 |
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| Main Authors: | , , , , , |
| Format: | pictorial research tables/charts Journal Article |
| Published: |
Wiley-Blackwell
Nov/Dec2016
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=120262922&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 120262922 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 10671927 DPV jtl: Wound Repair & Regeneration issn: 10671927 maglogo: Y pubinfo: dt: Nov/Dec2016 vid: 24 iid: 6 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 120262922 120262922 120262922 10.1111/wrr.12481 120262922 ppf: 1089 ppct: 8 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: An ex vivo porcine skin model to evaluate pressure-reducing devices of different mechanical properties used for pressure ulcer prevention. aug: au: Yeung, Ching‐Yan C. Holmes, David F. Thomason, Helen A. Stephenson, Christian Derby, Brian Hardman, Matthew J. affil: The Healing Foundation, Faculty of Life Sciences sug: subj: Pressure Ulcer Prevention and Control Bandages and Dressings Evaluation Mechanics Evaluation Models, Biological Product Evaluation Animal Studies Swine Funding Source Models, Anatomic Apoptosis Cytokines Metabolism Pressure Ulcer Physiopathology United Kingdom Human Compressive Strength Tensile Strength Fluorescent Antibody Technique Enzyme-Linked Immunosorbent Assay Descriptive Statistics Mann-Whitney U Test T-Tests One-Way Analysis of Variance Post Hoc Analysis P-Value Evaluation Research Skin Anatomy and Histology Comparative Studies Quantitative Studies ab: Pressure ulcers are complex wounds caused by pressure- and shear-induced trauma to skin and underlying tissues. Pressure-reducing devices, such as dressings, have been shown to successfully reduce pressure ulcer incidence, when used in adjunct to pressure ulcer preventative care. While pressure-reducing devices are available in a range of materials, with differing mechanical properties, understanding of how a material's mechanical properties will influence clinical efficacy remains limited. The aim of this study was to establish a standardized ex vivo model to allow comparison of the cell protection potential of two gel-like pressure-reducing devices with differing mechanical properties (elastic moduli of 77 vs. 35 kPa). The devices also displayed differing energy dissipation under compressive loading, and resisted strain differently under constant load in compressive creep tests. To evaluate biological efficacy we employed a new ex vivo porcine skin model, with a confirmed elastic moduli closely matching that of human skin (113 vs. 119 kPa, respectively). Static loads up to 20 kPa were applied to porcine skin ex vivo with subsequent evaluation of pressure-induced cell death and cytokine release. Pressure application alone increased the percentage of epidermal apoptotic cells from less than 2% to over 40%, and increased cellular secretion of the pro-inflammatory cytokine TNF-alpha. Co-application of a pressure-reducing device significantly reduced both cellular apoptosis and cytokine production, protecting against cellular damage. These data reveal new insight into the relationship between mechanical properties of pressure-reducing devices and their biological effects. After appropriate validation of these results in clinical pressure ulcer prevention with all tissue layers present between the bony prominence and external surface, this ex vivo porcine skin model could be widely employed to optimize design and evaluation of devices aimed at reducing pressure-induced skin damage. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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