Minimal Requirements for Primary HIV Latency Models Based on a Systematic Review.
Due to the scarcity of HIV-1 latently infected cells in patients, in vitro primary latency models are now commonly used to study the HIV-1 reservoir. To this end, a number of experimental systems have been developed. Most of these models differ based on the nature of the primary CD4 T-cell type, the...
| Publicado en: | AIDS Reviews Vol. 18; no. 4; pp. 171 - 184 |
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| Autores principales: | , , , |
| Formato: | Artículo |
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Publicidad Permanyer SLU
2016
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| Materias: | |
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=lth&AN=120267220&site=ehost-live header: @attributes: shortDbName: lth uiTerm: 120267220 longDbName: MedicLatina uiTag: AN controlInfo: bkinfo: jinfo: jid: 11396121 3PWJ jtl: AIDS Reviews issn: 11396121 maglogo: N pubinfo: dt: 2016 vid: 18 iid: 4 pid: 37650 pub: Publicidad Permanyer SLU artinfo: ui: 120267220 ppf: 171 ppct: 13 formats: fmt: @attributes: type: P size: 755KB tig: atl: Minimal Requirements for Primary HIV Latency Models Based on a Systematic Review. aug: au: Bonczkowski, Pawel De Scheerder, Marie-Angélique De Spiegelaere, Ward Vandekerckhove, Linos affil: HIV Translational Research Unit, Department of Internal Medicine, Faculty of Medicine and Health Sciences, Ghent University and Ghent University Hospital, Ghent, Belgium su: HIV infection transmission Systematic reviews CD4 antigen T cells PubMed (Online service) Viral latency-associated transcript protein sug: subj: HIV infection transmission Systematic reviews CD4 antigen T cells PubMed (Online service) Viral latency-associated transcript protein keyword: HIV-1 In vitro Model Primary cell Systematic review Viral latency ab: Due to the scarcity of HIV-1 latently infected cells in patients, in vitro primary latency models are now commonly used to study the HIV-1 reservoir. To this end, a number of experimental systems have been developed. Most of these models differ based on the nature of the primary CD4 T-cell type, the used HIV strains, activation methods, and latency assessment strategies. Despite these differences, most models share some common characteristics. Here, we provide a systematic review covering the primary HIV latency models that have been used to date with the aim to compare these models and identify minimal requirements for such experiments. A systematic search on PubMed and Web of Science databases generated a short list of 17 unique publications that propose new in vitro latency models. Based on the described methods, we propose and discuss a generalized workflow, visualizing all the necessary steps to perform such an in vitro study, with the key choices and validation steps that need to be made; from cell type selection until the model readout. pubtype: Academic Journal doctype: Article src: R language: English refInfo: copyright: @attributes: flag: Y custom: Copyright of AIDS Reviews is the property of Publicidad Permanyer SLU and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. item: AIDS Reviews holder: Publicidad Permanyer SLU dt: @attributes: year: 2016 holdings: @attributes: islocal: N |
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