Placenta Derived Mesenchymal Stem Cells Hosted on RKKP Glass-Ceramic: A Tissue Engineering Strategy for Bone Regenerative Medicine Applications.
In tissue engineering protocols, the survival of transplanted stem cells is a limiting factor that could be overcome using a cell delivery matrix able to support cell proliferation and differentiation. With this aim, we studied the cell-friendly and biocompatible behavior of RKKP glass-ceramic coate...
| Publicado en: | BioMed Research International Vol. 2016; pp. 1 - 12 |
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| Autores principales: | , , , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
12/19/2016
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=120287567&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 120287567 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 12/19/2016 vid: 2016 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 120287567 120287567 120287567 10.1155/2016/3657906 120287567 ppf: 1 ppct: 11 formats: fmt: @attributes: type: P tig: atl: Placenta Derived Mesenchymal Stem Cells Hosted on RKKP Glass-Ceramic: A Tissue Engineering Strategy for Bone Regenerative Medicine Applications. aug: au: Ledda, Mario Fosca, Marco De Bonis, Angela Curcio, Mariangela Teghil, Roberto Lolli, Maria Grazia De Stefanis, Adriana Marchese, Rodolfo Rau, Julietta V. Lisi, Antonella affil: Institute of Translational Pharmacology (IFT-CNR), Via del Fosso del Cavaliere 100, 00133 Rome, Italy sug: subj: Tissue Engineering Methods Placenta Stem Cells Bone Regeneration Titanium Glass Biocompatible Materials Materials Testing Human Cell Culture Techniques Amnion Cell Physiology Cytoskeletal Proteins Reverse Transcriptase Polymerase Chain Reaction Methods Staining and Labeling Cell Cycle Flow Cytometry Data Analysis Software In Vitro Studies T-Tests P-Value Descriptive Statistics Osteogenesis RNA Analysis Immunophenotyping Cell Differentiation Osteoblasts ab: In tissue engineering protocols, the survival of transplanted stem cells is a limiting factor that could be overcome using a cell delivery matrix able to support cell proliferation and differentiation. With this aim, we studied the cell-friendly and biocompatible behavior of RKKP glass-ceramic coated Titanium (Ti) surface seeded with human amniotic mesenchymal stromal cells (hAMSCs) from placenta. The sol-gel synthesis procedure was used to prepare the RKKP glass-ceramic material, which was then deposited onto the Ti surface by Pulsed Laser Deposition method. The cell metabolic activity and proliferation rate, the cytoskeletal actin organization, and the cell cycle phase distribution in hAMSCs seeded on the RKKP coated Ti surface revealed no significant differences when compared to the cells grown on the treated plastic Petri dish. The health of of hAMSCs was also analysed studying the mRNA expressions of MSC key genes and the osteogenic commitment capability using qRT-PCR analysis which resulted in being unchanged in both substrates. In this study, the combination of the hAMSCs’ properties together with the bioactive characteristics of RKKP glass-ceramics was investigated and the results obtained indicate its possible use as a new and interesting cell delivery system for bone tissue engineering and regenerative medicine applications. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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