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Antitumor Activity of Portulaca Oleracea L. Polysaccharide on HeLa Cells Through Inducing TLR4/NF-κB Signaling.

We have previously shown thatPortulaca oleraceaL. polysaccharide (POL-P3b) possesses the ability to inhibit cervical cancer cell growth in vitro and in vivo. In this study, we explored how toll-like receptor 4 (TLR4) signaling correlated with the antitumor mechanism of POL-P3b. Western blotting was...

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Published in:Nutrition & Cancer Vol. 69; no. 1; pp. 131 - 140
Main Authors: Zhao, Rui, Zhang, Tao, Ma, Baoling, Li, Xing
Format: equations & formulas research tables/charts Journal Article
Published: Taylor & Francis Ltd Jan2017
Online Access:View this record in EBSCOhost
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      dt: Jan2017
      vid: 69
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      pub: Taylor & Francis Ltd
      place: Philadelphia, Pennsylvania
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        120452780
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        10.1080/01635581.2017.1248294
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      tig:
        atl: Antitumor Activity of Portulaca Oleracea L. Polysaccharide on HeLa Cells Through Inducing TLR4/NF-κB Signaling.
      aug:
        au:
          Zhao, Rui
          Zhang, Tao
          Ma, Baoling
          Li, Xing
        affil: Department of Pharmaceutical Engineering, College of Life Science and Biotechnology, Heilongjiang Bayi Agricultural University, Daqing High-Tech Industrial Development Zone, Daqing, Heilongjiang, P.R. China
      sug:
        subj:
          Plants, Medicinal Therapeutic Use
          Polysaccharides
          Human
          Cell Physiology
          Intracellular Signaling Peptides and Proteins
          Cervix Neoplasms Prevention and Control
          Female
          In Vitro Studies
          In Vivo Studies
          Blotting, Western
          Biological Assay
          Receptors, Cell Surface
          Enzyme-Linked Immunosorbent Assay
          Immunity
          Cervix Neoplasms Diagnosis
          Cervix Neoplasms Physiopathology
          Cell Culture Techniques
          Flow Cytometry
          Descriptive Statistics
          Analysis of Variance
          Data Analysis Software
          T-Tests
          Apoptosis
          Funding Source
          Female
      ab: We have previously shown thatPortulaca oleraceaL. polysaccharide (POL-P3b) possesses the ability to inhibit cervical cancer cell growth in vitro and in vivo. In this study, we explored how toll-like receptor 4 (TLR4) signaling correlated with the antitumor mechanism of POL-P3b. Western blotting was utilized to detect the expression of TLR4 and the downstream signaling pathway. The level of inflammatory mediator was quantified using enzyme-linked immunosorbent assay (ELISA) kits. The effects of POL-P3b on the proliferation and apoptosis in HeLa cells were determined by WST-8 assay and Hoechst 33342/propidium iodide (PI) assay. Our results demonstrated that lipopolysaccharide (LPS) binding to TLR4 on tumor cells could enhance HeLa cell proliferation and increase the expression of TLR4 and the downstream molecules. Treating HeLa cells with POL-P3b could decrease the proliferation of HeLa cells, and upregulate Bax level and downregulate Bcl-2 level in a concentration-dependent manner. In addition, POL-P3b inhibited the protein expression levels of TLR4, MyD88, TRAF6, Activator Protein-1 (AP-1) and nuclear factor-κB (NF-κB) subunit P65 in HeLa cells. Furthermore, POL-P3b also reduced the production of cytokine/chemokine. Taken together, the present work suggested the antitumor mechanism of POL-P3b by downregulating TLR4 downstream signaling pathway and inducing cell apoptosis. Our results may provide direct evidence to suggest that POL-P3b should be considered as a potent nutrient supplement for oncotherapy.
      pubtype: Academic Journal
      doctype:
        equations & formulas
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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