Quantitative Analysis of [18F]FMISO PET for Tumor Hypoxia: Correlation of Modeling Results with Immunohistochemistry.
Purpose: Quantitative evaluation of tumor hypoxia based on H-1-(3-[18F]fluoro-2-hydroxypropyl)-2-nitroimidazole ([18F]FMISO) positron emission tomography (PET) can deliver important information for treatment planning in radiotherapy. However, the merits and limitations of different analysis methods...
| Publicado en: | Molecular Imaging & Biology Vol. 19; no. 1; pp. 120 - 130 |
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| Autores principales: | , , , , , , , , , , |
| Formato: | diagnostic images equations & formulas pictorial research tables/charts Journal Article |
| Publicado: |
Springer Nature
Feb2017
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=120531201&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 120531201 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 15361632 KJU jtl: Molecular Imaging & Biology issn: 15361632 maglogo: N pubinfo: dt: Feb2017 vid: 19 iid: 1 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 120531201 120531201 NLM27379986 120531201 10.1007/s11307-016-0975-4 NLM27379986 120531201 ppf: 120 ppct: 10 formats: fmt: @attributes: type: P tig: atl: Quantitative Analysis of [18F]FMISO PET for Tumor Hypoxia: Correlation of Modeling Results with Immunohistochemistry. aug: au: Shi, Kuangyu Bayer, Christine Astner, Sabrina Gaertner, Florian Vaupel, Peter Schwaiger, Markus Huang, Sung-Cheng Ziegler, Sibylle Astner, Sabrina T Gaertner, Florian C Ziegler, Sibylle I affil: Department of Nuclear Medicine , Klinikum rechts der Isar, Technische Universität München , Ismaningerstrasse. 22 81675 Munich Germany sug: subj: Tomography, Emission-Computed Methods Imidazoles Analogs and Derivatives Immunohistochemistry Imidazoles Cell Line, Tumor Mice Animal Studies ab: Purpose: Quantitative evaluation of tumor hypoxia based on H-1-(3-[18F]fluoro-2-hydroxypropyl)-2-nitroimidazole ([18F]FMISO) positron emission tomography (PET) can deliver important information for treatment planning in radiotherapy. However, the merits and limitations of different analysis methods in revealing the underlying physiological feature are not clear. This study aimed to assess these quantitative analysis methods with the support of immunohistological data.Procedures: Sixteen nude mice bearing xenografted human squamous cell carcinomas (FaDu or CAL-33) were scanned using 2-h dynamic [18F]FMISO PET. Tumors were resected and sliced, and the hypoxia marker pimonidazole was immunostained followed by H&E staining. The pimonidazole signal was segmented using a k-means clustering algorithm, and the hypoxic fraction (HF) was calculated as the hypoxic area/viable tumor-tissue-area ratio pooled over three tissue slices from the apical, center, and basal layers. PET images were analyzed using various methods including static analysis [standard uptake value (SUV), tumor-to-blood ratio (T/B), tumor-to-muscle ratio (T/M)] and kinetic modeling (Casciari αk A , irreversible and reversible two-tissue compartment k 3, Thorwarth w A k 3, Patlak K i , Logan V d , Cho K), and correlated with HF.Results: No significant correlation was found for static analysis. A significant correlation between k 3 of the irreversible two-tissue compartment model and HF was observed (r = 0.61, p = 0.01). The correlation between HF and αk A of the Casciari model could be improved through reducing local minima by testing more sets of initial values (r = 0.59, p = 0.02) or by reducing the model complexity by fixing three parameters (r = 0.63, p = 0.0008).Conclusions: With support of immunohistochemistry data, this study shows that various analysis methods for [18F]FMISO PET perform differently for assessment of tumor hypoxia. A better fitting quality does not necessarily mean a higher physiological correlation. Hypoxia PET analysis needs to consider both the mathematical stability and physiological fidelity. Based on the results of this study, preference should be given to the irreversible two-tissue compartment model as well as the Casciari model with reduced parameters. pubtype: Academic Journal doctype: diagnostic images equations & formulas pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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