Influence of Bovine Whey Protein Concentrate and Hydrolysate Preparation Methods on Motility in the Isolated Rat Distal Colon.
Whey protein concentrate (WPC) and hydrolysate (WPH) are protein ingredients used in sports, medical and pediatric formulations. Concentration and hydrolysis methods vary for whey sourced from cheese and casein co-products. The purpose of this research was to investigate the influence of whey proces...
| Publicado en: | Nutrients Vol. 8; no. 12; pp. 809 - 823 |
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| Autores principales: | , , , , , |
| Formato: | research tables/charts tracings Journal Article |
| Publicado: |
MDPI
Dec2016
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=120544513&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 120544513 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 20726643 B0TT jtl: Nutrients issn: 20726643 maglogo: N pubinfo: dt: Dec2016 vid: 8 iid: 12 pid: 97109 pub: MDPI artinfo: ui: 120544513 120544513 120544513 10.3390/nu8120809 120544513 ppf: 809 ppct: 14 formats: tig: atl: Influence of Bovine Whey Protein Concentrate and Hydrolysate Preparation Methods on Motility in the Isolated Rat Distal Colon. aug: au: Dalziel, Julie E. Anderson, Rachel C. Bassett, Shalome A. Lloyd-West, Catherine M. Haggarty, Neill W. Roy, Nicole C. affil: Food Nutrition & Health Team, Food & Bio-based Products Group, AgResearch, Grasslands Research Centre, Palmerston North 4442, New Zealand sug: subj: Gastrointestinal Motility Evaluation Milk Cheese Colon Functional Food Animal Studies Rats New Zealand In Vivo Studies In Vitro Studies Descriptive Statistics Data Analysis Software Naloxone Analysis of Variance Post Hoc Analysis Repeated Measures Confidence Intervals Flow Cytometry Funding Source Male Adult Adult: 19-44 years Male ab: Whey protein concentrate (WPC) and hydrolysate (WPH) are protein ingredients used in sports, medical and pediatric formulations. Concentration and hydrolysis methods vary for whey sourced from cheese and casein co-products. The purpose of this research was to investigate the influence of whey processing methods on in vitro gastrointestinal (GI) health indicators for colonic motility, epithelial barrier integrity and immune modulation. WPCs from casein or cheese processing and WPH (11% or 19% degree of hydrolysis, DH) were compared for their effects on motility in a 1 cm section of isolated rat distal colon in an oxygenated tissue bath. Results showed that WPC decreased motility irrespective of whether it was a by-product of lactic acid or mineral acid casein production, or from cheese production. This indicated that regardless of the preparation methodology, the whey protein contained components that modulate aspects of motility within the distal colon. WPH (11% DH) increased contractile frequency by 27% in a delayed manner and WPH (19% DH) had an immediate effect on contractile properties, increasing tension by 65% and frequency by 131%. Increased motility was associated with increased hydrolysis that may be attributed to the abundance of bioactive peptides. Increased frequency of contractions by WPH (19% DH) was inhibited (by 44%) by naloxone, implicating a potential involvement of opioid receptors in modulation of motility. Trans-epithelial electrical resistance and cytokine expression assays revealed that the WPC proteins studied did not alter intestinal barrier integrity or elicit any discernible immune response. pubtype: Academic Journal doctype: research tables/charts tracings Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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