Proliferative and Invasive Effects of Progesterone-Induced Blocking Factor in Human Glioblastoma Cells.
Progesterone-induced blocking factor (PIBF) is a progesterone (P4) regulated protein expressed in different types of high proliferative cells including astrocytomas, the most frequent and aggressive brain tumors. It has been shown that PIBF increases the number of human astrocytoma cells. In this wo...
| Publicado en: | BioMed Research International Vol. 2017; pp. 1 - 11 |
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| Autores principales: | , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
1/12/2017
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=120681789&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 120681789 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 1/12/2017 vid: 2017 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 120681789 120681789 120681789 10.1155/2017/1295087 120681789 ppf: 1 ppct: 10 formats: fmt: @attributes: type: P tig: atl: Proliferative and Invasive Effects of Progesterone-Induced Blocking Factor in Human Glioblastoma Cells. aug: au: Gutiérrez-Rodríguez, Araceli Hansberg-Pastor, Valeria Camacho-Arroyo, Ignacio affil: Unidad de Investigación en Reproducción Humana, Instituto Nacional de Perinatología-Facultad de Química, Universidad Nacional Autónoma de México (UNAM), Ciudad Universitaria, Coyoacán, 04510 Ciudad de México, Mexico sug: subj: Glioma Pathology Cell Physiology Evaluation Progesterone Analysis Immunologic Factors Analysis Human Cell Culture Techniques Cell Line, Tumor Cell Migration Assays Neoplasm Invasiveness Receptors, Cytoplasmic and Nuclear Antagonists and Inhibitors Wound Healing Biological Assay RNA Analysis Reverse Transcriptase Polymerase Chain Reaction Blotting, Western Genetic Techniques Descriptive Statistics One-Way Analysis of Variance Data Analysis Software Two-Way Analysis of Variance P-Value T-Tests Post Hoc Analysis Gene Expression Funding Source ab: Progesterone-induced blocking factor (PIBF) is a progesterone (P4) regulated protein expressed in different types of high proliferative cells including astrocytomas, the most frequent and aggressive brain tumors. It has been shown that PIBF increases the number of human astrocytoma cells. In this work, we evaluated PIBF regulation by P4 and the effects of PIBF on proliferation, migration, and invasion of U87 and U251 cells, both derived from human glioblastomas. PIBF mRNA expression was upregulated by P4 (10 nM) from 12 to 24 h. Glioblastoma cells expressed two PIBF isoforms, 90 and 57 kDa. The content of the shorter isoform was increased by P4 at 24 h, while progesterone receptor antagonist RU486 (10 μM) blocked this effect. PIBF (100 ng/mL) increased the number of U87 cells on days 4 and 5 of treatment and induced cell proliferation on day 4. Wound-healing assays showed that PIBF increased the migration of U87 (12–48 h) and U251 (24 and 48 h) cells. Transwell invasion assays showed that PIBF augmented the number of invasive cells in both cell lines at 24 h. These data suggest that PIBF promotes proliferation, migration, and invasion of human glioblastoma cells. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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