Cover Image
QR Code

مقایسه سه روش PCR، کشت و اوره آز سریع در تشخیص هلیکوباکتر پیلوری از نمونه بیوپسی معده

Background & Objectives: Helicobacter pylori prevalence is high in developing countries. This microorganism is accepted as the most important agent of gastritis and as a risk factor for peptic ulcer disease and gastric adenocarcinoma. Currently many diagnostic methods exist for detecting H. pylori,...

Full description

Published in:Medical Journal of Tabriz University of Medical Sciences & Health Services Vol. 38; no. 5; pp. 70 - 76
Main Authors: نفیسی, محمدرضا, علیپور شادباد, محمد, رحیمیان, قربانعلی, کریمی, علی
Format: pictorial research tables/charts Journal Article
Published: Tabriz University of Medical Sciences Dec2016-Jan2017
Online Access:View this record in EBSCOhost
fields @attributes:
  recordID: 1
pdfLink:
plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=120715141&site=ehost-live
header:
  @attributes:
    shortDbName: ccm
    uiTerm: 120715141
    longDbName: CINAHL Complete
    uiTag: AN
  controlInfo:
    bkinfo:
    dissinfo:
    jinfo:
      jid:
        16085671
        EUW8
      jtl: Medical Journal of Tabriz University of Medical Sciences & Health Services
      issn: 16085671
      maglogo: N
    pubinfo:
      dt: Dec2016-Jan2017
      vid: 38
      iid: 5
      pid: 69573
      pub: Tabriz University of Medical Sciences
    artinfo:
      ui:
        120715141
        120715141
        120715141
        120715141
      ppf: 70
      ppct: 6
      formats:
        fmt:
          @attributes:
            type: P
      tig:
        atl: مقایسه سه روش PCR، کشت و اوره آز سریع در تشخیص هلیکوباکتر پیلوری از نمونه بیوپسی معده
      aug:
        au:
          نفیسی, محمدرضا
          علیپور شادباد, محمد
          رحیمیان, قربانعلی
          کریمی, علی
        affil: مرکز تحقیقات سلولی و مولکولی، دانشگاه علوم پزشکی شهرکرد، شهرکرد، ایران
      sug:
      ab: Background & Objectives: Helicobacter pylori prevalence is high in developing countries. This microorganism is accepted as the most important agent of gastritis and as a risk factor for peptic ulcer disease and gastric adenocarcinoma. Currently many diagnostic methods exist for detecting H. pylori, however they all have limitations. The aim of this descriptive study was to evaluate diagnostic methods, such as rapid urease test, culture and polymerase chain reaction (PCR). Materials and Methods: For identification of Helicobacter pylori 100 patients (46 women and 54 men) who were suffering from digestive complaints and referred to the endoscopy department of Hajar Hospital in Sharkord were participated in the study. Gastric biopsy samples were collected from each patient, then polymerase chain reaction, culture and rapid urease test were performed. DNA extraction was followed by PCR was used for diagnosis of ureC gene. Results: Biopsy samples from100 patients were evaluated. H. pylori was positively identified by PCR in 78/100 (78%) of the patients, while positive samples were found in 52 (52%) and 48 (48%) of the patients by RUT and culture methods, respectively. In results of the culture, there was an agreement of 100% with PCR. Conclusion: Three different methods for the detection of Helicobacter pylori were evaluated. Given that the PCR test has higher sensitivity and specificity to detect H.pylori comparing rapid urea as test, therefore this method could be used to detect H.pylori.
      pubtype: Academic Journal
      doctype:
        pictorial
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: Persian
    refInfo:
    holdings:
      @attributes:
        islocal: N