The effect of potassium diclofenac and dexamethasone on MMP-1 gene transcript levels during experimental tooth movement in rats.

Objectives: The aim of this study was to analyze MMP-1 transcript levels in periodontal tissues of rats that underwent orthodontic treatment using potassium diclofenac and dexamethasone at different stages of tooth movement.Setting and Sample Population: The sample comprised of ninety male Wistar ra...

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Publicado en:Orthodontics & Craniofacial Research Vol. 20; no. 1; pp. 30 - 35
Autores principales: Molina Da Silva, G. P., Tanaka, O. M., Campos Navarro, D. F., Repeke, C. E., Garlet, G. P., Guariza‐Filho, O., Trevilatto, P. C.
Formato: pictorial research tables/charts Journal Article
Publicado: Wiley-Blackwell Feb2017
Acceso en línea:Ver este registro en EBSCOhost
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      dt: Feb2017
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      pub: Wiley-Blackwell
      place: Malden, Massachusetts
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        120786328
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        10.1111/ocr.12137
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        120786328
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        atl: The effect of potassium diclofenac and dexamethasone on MMP-1 gene transcript levels during experimental tooth movement in rats.
      aug:
        au:
          Molina Da Silva, G. P.
          Tanaka, O. M.
          Campos Navarro, D. F.
          Repeke, C. E.
          Garlet, G. P.
          Guariza‐Filho, O.
          Trevilatto, P. C.
        affil: School of Life Sciences Curitiba, Pontifícia Universidade Católica do Paraná, Curitiba Paraná, Brazil
      sug:
        subj:
          Antiinflammatory Agents Pharmacodynamics
          Peptide Hydrolases Drug Effects
          Dexamethasone Pharmacodynamics
          Diclofenac Pharmacodynamics
          Peptide Hydrolases Metabolism
          Periodontium Drug Effects
          Rats
          RNA Metabolism
          RNA Drug Effects
          Periodontium Metabolism
          Animal Studies
      ab: Objectives: The aim of this study was to analyze MMP-1 transcript levels in periodontal tissues of rats that underwent orthodontic treatment using potassium diclofenac and dexamethasone at different stages of tooth movement.Setting and Sample Population: The sample comprised of ninety male Wistar rats.Material and Methods: A closed nickel-titanium coil spring was used to apply a force of 50 cN to move the maxillary right first molars mesially. One group received daily doses of 0.9% saline solution, the second group received daily doses of 5 mg/kg potassium diclofenac, and the third group received daily doses of 0.5 mg/kg dexamethasone. Tooth movement was observed on days 0, 1, 3, 7, and 14. MMP-1 transcript levels were evaluated by real-time polymerase chain reaction and the results were compared between groups by three-way ANOVA, with a significance level of 0.05.Results: Transcript levels increased in groups that received the coil spring treatment on all days of the experiment. MMP-1 expression was found to be decreased in groups treated with potassium diclofenac and dexamethasone compared to that in the control group, on days 1, 3, 5, and 7.Conclusions: The application of orthodontic forces significantly increased MMP-1 transcript levels. The use of anti-inflammatory drugs may have an inhibitory effect on MMP-1 expression.
      pubtype: Academic Journal
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      ougenre: Article
    language: English
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